Tags: fluorescence imaging

Description

Fluorescence imaging/Microscopy is the non-evasive visualization of fluorescent dyes or proteins as labels for molecular processes or structures. Images can be produced from a variety of methods including: microscopy, imaging probes, and spectroscopy. This type of imaging enables a wide range of experimental observations including the location and dynamics of gene expression, protein expression and molecular interactions in cells and tissues.

More information see: Wikipedia

All Categories (1-20 of 26)

  1. Aggregation Effect of Near-Infrared Boron Difluoride Formazanate Dyes

    21 Aug 2022 | | Contributor(s):: Jessica Robicheaux, Rice University

    How are near-infrared (NIR) dyes used for their enhanced capability in fluorescence detection and imaging, tissue penetration depth and low background with minimal fluorescence from surrounding tissues? This research focuses on Aggregation Induced Emission (AIE) in order to utilize boron...

  2. Photosynthetic Protein Spectroscopy Lab

    19 Oct 2020 | | Contributor(s):: Safa Ahad, Chientzu Lin, Michael Earl Reppert

    This tool parses PDB structures of photosynthetic proteins to identify pigments, calculate inter-pigment interactions, and simulate optical spectra for the complex.

  3. Extracting Hidden Three-Dimensional Information from Your Fluorescence Microscope

    06 Mar 2019 | | Contributor(s):: Mikael Backlund

    Here I will focus on two studies that demonstrate this concept. First I will discuss the fundamental limits associated with determining the three-dimensional position of a fluorescent emitter, with insights provided by quantum parameter estimation theory. Second I will show how nitrogen-vacancy...

  4. The Use of Probes, Beams, and Waves for Characterization at the Nano-Scale

    20 Jul 2018 | | Contributor(s):: Stephen J. Fonash, NACK Network

    OutlineThe tools for characterizing materials and structures at the nano-scaleProbesPhoton beamsElectron beamsIon beamsAcoustic waves

  5. Philippe Legros

    https://nanohub.org/members/160703

  6. [Illinois] AMC 2016 workshop: An introduction to elemental analysis by ED-XRF and an overview of its applications to materials characterization laboratories

    13 Jun 2016 | | Contributor(s):: Justin Masone

    Advanced Material Characterization Workshop June 7-8 2016 Federick Seitz Materials Research Laboratory, University of Illinois at Urbana Champaign  

  7. [Illinois] PHYS 598BP Lecture 6: FIONA and STORM

    06 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  8. [Illinois] Physics 598BP Lecture 9: FIONA III: GFPs

    06 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  9. [Illinois] PHYS 598BP Lecture 10: FIONA IV

    03 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  10. [Illinois] PHYS 598BP Lecture 2: Fluorescence, Lifetimes and FRET (Lab 1)

    11 Apr 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  11. [Illinois] ECE 564: Modern Light Microscopy

    11 Apr 2016 | | Contributor(s):: Gabriel Popescu

    Current research topics in modern light microscopy: optics principles (statistical optics, Gaussian optics, elastic light scattering, dynamic light scattering); traditional microscopy (bright field, dark field, DIC, phase contract, confocal, epi-fluorescence, confocal fluorescence); current...

  12. [Illinois] Biophysics 401 Lecture 19: Fluorescence Microscopy & Imaging

    10 Nov 2015 | | Contributor(s):: Paul R Selvin

  13. [Illinois] Biophysics 401 Lecture 18: Fluorescence

    05 Nov 2015 | | Contributor(s):: Paul R Selvin

  14. [Illinois] Biophotonics 2012: Single Molecule Fluorescence and Optical Trapping Applied to Molecular Motors: Two Can Do it Better Than One

    06 Jun 2012 | | Contributor(s):: Paul R Selvin

  15. [Illinois] Biophotonics 2012: Photonic Crystal Enhanced Fluorescence Imaging

    06 Jun 2012 | | Contributor(s):: meng lu

  16. [Illinois] Biophotonics 2012: Basic Principles of Fluorescence

    23 May 2012 | | Contributor(s):: Beniamino Barbieri

  17. [Illinois] BioE 498/ME 498: Experimental Methods for Biological Machines (Traction Force Microscopy Experiment)

    09 May 2012 | | Contributor(s):: Taher A. Saif, Samantha Knoll, Sandeep Anand

    Traction Force Microscopy (TFM)Using polyacrylamide gels with fluorescent microspheres, students in Professor Saif's laboratory Samantha and Sandeep show how to track cellular motion and calculate traction forces. In this video, the students first walk through the procedure of creating the...

  18. Illinois Tool: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

    01 Nov 2010 | | Contributor(s):: Paul R Selvin, Raheem Syed, Nahil Sobh

    Fluorescence Imaging with One Nanometer Accuracy

  19. Illinois iOptics Lecture 1: Super Accuracy and Super-Resolution of Molecular Motors and Ion Channels

    15 Apr 2010 | | Contributor(s):: Paul R Selvin

    The standard diffraction limit of light is about 250 nm, meaning that you cannot "resolve" objects closer than this distance. Despite this, we have come up with a method to measure individual biomolecules with 1.5 nm spatial localization in x-y plane and 1-500 msec temporal resolution,...

  20. Illinois Phys550 Molecular Biophysics Lecture 13: Fluorescence Energy Transfer and Light Harvesting in Photosynthesis

    10 Mar 2010 | | Contributor(s):: Klaus Schulten