Tags: Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

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Description

Fluorescence Imaging with One-Nanometer Accuracy (FIONA) is a technique for localizing a single dye, or a single group of dyes, to within ~1-nm accuracy. This high degree of precision is achieved using total internal reflection fluorescence microscopy, deoxygenation agents, and a high quantum yield, low-noise detector.

For nanoHUB Simulation see: FIONA

Resources (1-13 of 13)

  1. [Illinois] PHYS 598BP Lecture 7: More FIONA

    14 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  2. [Illinois] PHYS 598BP Lecture 6: FIONA and STORM

    06 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  3. [Illinois] Physics 598BP Lecture 9: FIONA III: GFPs

    06 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  4. [Illinois] PHYS 598BP Lecture 10: FIONA IV

    03 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  5. [Illinois] ECE 564: Modern Light Microscopy

    11 Apr 2016 | | Contributor(s):: Gabriel Popescu

    Current research topics in modern light microscopy: optics principles (statistical optics, Gaussian optics, elastic light scattering, dynamic light scattering); traditional microscopy (bright field, dark field, DIC, phase contract, confocal, epi-fluorescence, confocal fluorescence); current...

  6. Illinois Tool: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

    01 Nov 2010 | | Contributor(s):: Paul R Selvin, Raheem Syed, Nahil Sobh

    Fluorescence Imaging with One Nanometer Accuracy

  7. Illinois iOptics Lecture 1: Super Accuracy and Super-Resolution of Molecular Motors and Ion Channels

    15 Apr 2010 | | Contributor(s):: Paul R Selvin

    The standard diffraction limit of light is about 250 nm, meaning that you cannot "resolve" objects closer than this distance. Despite this, we have come up with a method to measure individual biomolecules with 1.5 nm spatial localization in x-y plane and 1-500 msec temporal resolution,...

  8. Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

    out of 5 stars 

    11 Apr 2008 | | Contributor(s):: Paul R Selvin

    Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability

  9. Lecture 12: Ultra-Resolution

    out of 5 stars 

    11 Apr 2008 | | Contributor(s):: Paul R Selvin

    SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization Microscopy), Enhancing Resolution

  10. Lecture 13: Enhancing Resolution - FIONA - SHREC - DOPI - PALM - STORM

    out of 5 stars 

    15 Apr 2008 | | Contributor(s):: Paul R Selvin

    Current Methods of obtaining higher resolution using: FIONA : Flouresence Imaging with One Nanometer Accuracy SHREC : Single molecule High Resolution Co-localization DOPI : Defocused Orientation Position Imaging

  11. Lecture 9: X-ray Structure and FIONA

    out of 5 stars 

    07 Apr 2008 | | Contributor(s):: Paul R Selvin

    Accuracy vs. Resolution Measuring atomic distances Biomolecular Motors: Intra- AND Extra-Cellular Motion

  12. Lecture 10: Mutagenesis

    out of 5 stars 

    07 Apr 2008 | | Contributor(s):: Paul R Selvin

    Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)

  13. Illinois Physics 498: Introduction to Biological Physics

    out of 5 stars 

    07 Apr 2008 | | Contributor(s):: Paul R Selvin

    We will apply simple yet powerful ideas of physics to gain some understanding of biology. (What is the inertia of a bacteria and how does this affect its behavior?) We will begin with atoms, move to molecules, then macromolecules, then cells, and finally whole systems. For example, how do we...