Lecture 21: Nerves
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24 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Ion Channels,Ionic current, Gating current, Digital Ion Channels, Structural studies, X-ray Crystallography
Lecture 20: Diffusion - Part2
23 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Diffusion and bacteria moving, power consumed by bacteria, Introduction to Reynolds number, Where Bacteria Live, How E. Coli move and swim,
Lecture 19: Optical Traps - Part2
Biological application of optical traps, High resolution optical trapping, Brownian noise
Lecture 16: Optical Traps - Part 1
16 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
First Optical Trap built, Reflection, Refraction, Brownian motionYann Chemla - Assistant Professor of Physics - University of Illinois at Champaign-Urbana
Lecture 17: Diffusion - Part 1
15 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Diffusion, Directed motors, Thermal motion, nerve synapse, Efficiency of Diffusion
Lecture 15: Confocal and STED Microscopy
Confocal Detection, Energy Transfer, Confocal Microscopy, STED (Stimulated Emission Depletion),Improved resolution
Lecture 14: FRET and Helicase Activity
FRET: measuring conformational changes of (single) biomolecules, Unzipping mystery of helicases
Lecture 13: Enhancing Resolution - FIONA - SHREC - DOPI - PALM - STORM
Current Methods of obtaining higher resolution using: FIONA : Flouresence Imaging with One Nanometer Accuracy SHREC : Single molecule High Resolution Co-localization DOPI : Defocused Orientation Position Imaging
Lecture 12: Ultra-Resolution
11 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization Microscopy), Enhancing Resolution
Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)
Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability
Lecture 10: Mutagenesis
07 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)
Lecture 9: X-ray Structure and FIONA
Accuracy vs. Resolution Measuring atomic distances Biomolecular Motors: Intra- AND Extra-Cellular Motion
Lecture 8: Resolutions
X-ray diffraction (atomic resolution)Electron (Imaging) Microscopy (nm-scale)Visible (Imaging) Microscopy (nm - µm)
Lecture 7: Single-Molecule of ATPase
ATPase - How it produces ATP?
Lecture 6: Magnetic Tweezers
Introduction II and Applications
Lecture 5: Magnetic Traps & DNA
Introduction I
Illinois Physics 498: Introduction to Biological Physics
07 Apr 2008 | Courses | Contributor(s): Paul R Selvin
We will apply simple yet powerful ideas of physics to gain some understanding of biology. (What is the inertia of a bacteria and how does this affect its behavior?) We will begin with atoms, move to molecules, then macromolecules, then cells, and finally whole systems. For example, how do we...
Lecture 4 : Applications of DNA Technology: FISH, PCR, Forensics
06 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
FISH (Florescence In Situ Hybridization), Gene Arrays("Chips") can be made
Lecture 3: Nucleic Acids, RNA, and Proteins
11 Feb 2008 | Online Presentations | Contributor(s): Paul R Selvin
Nucleic Acids, Proteins, DNA Dimensions and Stability, How to make a nucleotide