[Illinois] Chemical and Biological Detection for Diagnostic, Food Safety, and Drug Safety using the Internal Sensing Capabilities of Mobile Devices
Online Presentations | 20 Jul 2016 | Contributor(s): Brian Cunningham
The overall objective of the 2015 BioNanotechnology Summer Institute is to enhance the ability to address challenges in Cancer Nanotechnology and Mechanobiology, offering expertise from interdisciplinary faculty in biology, engineering, materials science, and nanotechnology, through a combination...
[Illinois] Optics-based Biosensors for Diagnostics, Cell Imaging, Pharmaceutical Research, and Tissue Imaging
Online Presentations | 28 Aug 2013 | Contributor(s): Brian Cunningham
[Illinois] ECE 416 Guest Speaker Dr. Greg Underhill
Online Presentations | 14 May 2013 | Contributor(s): Brian Cunningham, George Daley, Greg Underhill
In this lecture, Dr. Greg Underhill explains the relations of stem cell fate in correspondence to biosensors. The lecture starts out with a brief introduction of stem cells and a liver and pancreas differentiation. In the endoderm,...
[Illinois] ECE 416 Nanoparticles
Online Presentations | 17 May 2013 | Contributor(s): Brian Cunningham
In this lecture, we discussed the concept of nanoparticles and quantum dots. A nanoparticle has a solid object size and can be suspended in a solution. It is visualized with light by observation of fluorescence or absorption. Its...
[Illinois] ECE 416 Guest Speaker Dr. Sua Myong
Online Presentations | 14 May 2013 | Contributor(s): Su-A Myong, Brian Cunningham, George Daley
In this lecture, Single Molecule Studies of Rad51 and Srs2 will be discussed by Dr. Myong. We first look at the different ways to carry out the single molecule fluorescence detection through colors. The way the signals are...
[Illinois] ECE 416 Surface Enhanced Raman Spectroscopy II
Online Presentations | 14 May 2013 | Contributor(s): Brian Cunningham
In this lecture, we continued our discussion on Surface Enhanced Raman Spectroscopy (SERS). We started off with a revision of the previous lecture and focused on the important concepts like the two important wavelengths: the...
[Illinois] ECE 416 DNA MicroArrays II
In this lecture, we start off with a revision of the previous lecture. The Microarray scanner is used to read the detection and build an xy image of the fluorescent intensity. The system has at least two lasers, one for each dye....
[Illinois] ECE 416 Surfance Enhanced Raman Spectroscopy I
Online Presentations | 09 May 2013 | Contributor(s): Brian Cunningham, NanoBio Node
In this lecture, we started with the concept of a surface enhanced raman spectroscopy. It was observed that it is a powerful measurement technique, but the Raman scattering is a very weak effect. We see that the surface plays a...
[Illinois] ECE 416 Lecture 36: Raman Spectroscopy
Online Presentations | 09 May 2013 | Contributor(s): Guillermo Monroy, Brian Cunningham
Learn the underlying engineering principles used to detect small molecules, DNA, proteins, and cells in the context of applications in diagnostic testing, pharmaceutical research, and environmental monitoring. Biosensor approaches including electrochemistry, fluorescence, acoustics, and optics...
[Illinois] ECE 416 Next Gen Sequencing II
In this lecture, we start off with a revision of the Illumina company and their DNA sequencing technology. After that we move on to the Helicos Company and their true Single Molecule Sequencing (tSMS). It starts off with a glass...
[Illinois] ECE 416 Protein Microarrays II, Next Gen Sequencing I
In this lecture, we start off by finishing the lecture of protein microarrays. We conclude with the practical issues and problems including the capture molecule not behaving uniformly and the antibody/antigen binding conditions are...
[Illinois] ECE 416 Protein Microarrays I
In this lecture, we discussed the concepts of a Protein Microarray. The goal would be to be able to detect individual proteins. In the microarray, there are simultaneous measurements of many proteins for a small volume. A panel of...
[Illinois] ECE 416 DNA Microarrays I
Online Presentations | 08 May 2013 | Contributor(s): Brian Cunningham
In this lecture, we started off with a revision of DNA with genes and gene expressions and how DNA sends messages. The problem with gene detection is how to analyze expressions with thousands of genes and determine what possible...
[Illinois] ECE 416 Surface Functionalization II
Online Presentations | 13 May 2013 | Contributor(s): Brian Cunningham
In this lecture, we start off the discussion talking about the different functional groups. We see that the amines as bases and that carboxyl groups acts as acids. These two groups then covalently bond and eliminate H2O. Another...
[Illinois] ECE 416 Avidin-Biotin and Surface Functionalization I
In this lecture, we start off by taking a look at X-Ray Crystallography and how it is used to understand how biomolecules binding works. The x-ray data is processed by computer algorithms to determine coordinates of all atoms in...
[Illinois] ECE 416 Fluorescent Beads
In this lecture, we start off talking about the luminex company and their new technology of fluorescent beads and the things it can be used for. It allows for the detection of multiple of allergies, cancer cells, and heart...
[Illinois] ECE 416 Homogeneous Assays II
In this lecture, we start with a review of the previous lecture and fluorescence polarization and dyes on homogeneous assays. The properties of fluorescent dyes are then briefly glanced over again which include absorption,...
[Illinois] ECE 416 Fluorescence III and Homogeneous Assays I
In this lecture, we continue the discussion of Fluorescence. Examples of results are shown from the fluorescent dyes and how they may be used from a fluorescent microscope. Getting results and images from Fluorescence Scanners are...
[Illinois] ECE 416 Fluorescence II
Online Presentations | 10 Apr 2013 | Contributor(s): Brian Cunningham
In this lecture, we start with a revision of the common organic dyes and the photostability and quenching concepts. Quenching is then discussed in more detail and the difference between self-quenching and external quenching....
[Illinois] ECE 416 Fluorescence I
In this lecture, we discussed Fluorescence molecules and how they can be used for detection of other molecules. The molecules are considered to have high energy states, which helps them with their detection. The photons discussed...
[Illinois] ECE 416 Lumerical Optical Simulation
In this lecture, we looked at lumerical optical simulation. The motivation to pursue this topic is because there is now software available that allows us to conduct simulations to visualize Electromagnetic field locations, to...
[Illinois] ECE 416 Impedance Based Sensors II
In this lecture, we start off with a light review of the nanopore sequencing method which is based upon having a carefully bioengineered pore that can induce different changes in charge blockages as different base pairs go through...
[Illinois] ECE 416 Impedance Based Sensors I
Online Presentations | 27 Mar 2013 | Contributor(s): Brian Cunningham
In this lecture, we were introduced to the concept of a new sensor called the impedance-based Biosensor. Biomolecules are originally charged and therefore there is a need to determine the charge of the molecule before using it on a...
[Illinois] ECE 416 Optical Sensors III
In this lecture, we finished our discussion over optical sensors. We started with discussing a photon moving between 2 mirrors with 100% efficiency, the photon gets to "sample" the media between the mirrors with no loss. This was...
[Illinois] ECE 416 Optical Sensors II
In this lecture, there is a review of the previous lecture of SPR Sensors along with Reflection Interference Spectroscopy. We learned that as we go to longer wavelengths, the refractive index is more constant. Then, we looked at...