Tags: Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

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Description

Fluorescence Imaging with One-Nanometer Accuracy (FIONA) is a technique for localizing a single dye, or a single group of dyes, to within ~1-nm accuracy. This high degree of precision is achieved using total internal reflection fluorescence microscopy, deoxygenation agents, and a high quantum yield, low-noise detector.

For nanoHUB Simulation see: FIONA

Online Presentations (1-10 of 10)

  1. [Illinois] PHYS 598BP Lecture 7: More FIONA

    Online Presentations | 14 Jun 2016 | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  2. [Illinois] PHYS 598BP Lecture 6: FIONA and STORM

    Online Presentations | 06 Jun 2016 | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  3. [Illinois] Physics 598BP Lecture 9: FIONA III: GFPs

    Online Presentations | 06 Jun 2016 | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  4. [Illinois] PHYS 598BP Lecture 10: FIONA IV

    Online Presentations | 03 Jun 2016 | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  5. Illinois iOptics Lecture 1: Super Accuracy and Super-Resolution of Molecular Motors and Ion Channels

    Online Presentations | 16 Apr 2010 | Contributor(s):: Paul R Selvin

    The standard diffraction limit of light is about 250 nm, meaning that you cannot "resolve" objects closer than this distance. Despite this, we have come up with a method to measure individual biomolecules with 1.5 nm spatial localization in x-y plane and 1-500 msec temporal resolution,...

  6. Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

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    Online Presentations | 11 Apr 2008 | Contributor(s):: Paul R Selvin

    Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability

  7. Lecture 12: Ultra-Resolution

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    Online Presentations | 11 Apr 2008 | Contributor(s):: Paul R Selvin

    SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization Microscopy), Enhancing Resolution

  8. Lecture 13: Enhancing Resolution - FIONA - SHREC - DOPI - PALM - STORM

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    Online Presentations | 15 Apr 2008 | Contributor(s):: Paul R Selvin

    Current Methods of obtaining higher resolution using: FIONA : Flouresence Imaging with One Nanometer Accuracy SHREC : Single molecule High Resolution Co-localization DOPI : Defocused Orientation Position Imaging

  9. Lecture 9: X-ray Structure and FIONA

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    Online Presentations | 07 Apr 2008 | Contributor(s):: Paul R Selvin

    Accuracy vs. Resolution Measuring atomic distances Biomolecular Motors: Intra- AND Extra-Cellular Motion

  10. Lecture 10: Mutagenesis

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    Online Presentations | 07 Apr 2008 | Contributor(s):: Paul R Selvin

    Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)