[Illinois] Phys550 Lecture 23: Single Molecular Manipulation & Optical Traps

By Yann Chamla

University of Illinois at Urbana-Champaign

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Researchers should cite this work as follows:

  • Yann Chamla (2013), "[Illinois] Phys550 Lecture 23: Single Molecular Manipulation & Optical Traps," https://nanohub.org/resources/19777.

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University of Illinois at Urbana-Champaign, Urbana, IL

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NanoBio Node

University of Illinois at Urbana-Champaign

[Illinois] Phys550 Lecture 23: Single Molecular Manipulation & Optical Traps
by: Yann Chamla
  • Single Molecular Manipulation by Optical Traps 1. Single Molecular Manipulation … 0
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  • Introduction 2. Introduction 61.849516939327579
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  • The Cellular 3. The Cellular "Factory" 87.7679919606277
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  • Molecular Machines 4. Molecular Machines 173.55252647581747
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  • Molecular Machines 5. Molecular Machines 217.68445463681104
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  • Molecular Machines 6. Molecular Machines 347.47695122265452
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  • Principles of Optical Trapping 7. Principles of Optical Trapping 397.55925171996182
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  • First Optical Trap 8. First Optical Trap 472.06287201422356
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  • Optical Forces 9. Optical Forces 555.73998299363552
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  • First 10. First "Gradient" Optical Trap 702.01996959468158
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  • Optical 11. Optical "Tweezers" 740.44945760004123
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  • Quantitative Optical Traps 12. Quantitative Optical Traps 909.66317091396331
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  • Optical Detection 13. Optical Detection 965.69584374758426
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  • Calibration 14. Calibration 1080.3644773119638
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  • Power Spectrum 15. Power Spectrum 1191.4380942564869
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  • Infrared Traps Support Life! 16. Infrared Traps Support Life! 1421.6431240176248
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  • IR Traps and Photodamage 17. IR Traps and Photodamage 1443.4611559174418
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  • Tethering Geometries 18. Tethering Geometries 1497.2624391249451
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  • Information Extraction 19. Information Extraction 1736.1450952098739
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  • Example Application of Optical Traps 20. Example Application of Optical… 1762.921770723286
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  • Optical Trap Layout 21. Optical Trap Layout 1782.3844469066453
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  • The Optical Trap 22. The Optical Trap 1851.0616609549331
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  • Example Experiment 23. Example Experiment 1886.2680306114562
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  • Traps in Action 24. Traps in Action 2029.3248988636656
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  • DNA Hairpin 25. DNA Hairpin 2239.9432863511042
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  • Current State-of-the-Art Optical Traps 26. Current State-of-the-Art Optic… 2392.6695096498233
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  • Resolution in Traps 27. Resolution in Traps 2657.9569429771441
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  • High-Resolution Traps 28. High-Resolution Traps 2783.658558581772
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  • XPD Helicase 29. XPD Helicase 2845.8895359323865
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  • DNA Helicase Mechanism 30. DNA Helicase Mechanism 2952.004509263315
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  • Hairpin Assay 31. Hairpin Assay 3029.7312479064135
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  • Laminar Flow Chambers 32. Laminar Flow Chambers 3137.3338143214205
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  • Single Helicase Unwinding 33. Single Helicase Unwinding 3215.3084851451981
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  • Stepping Dynamics & Sequence 34. Stepping Dynamics & Sequence 3323.5308820119049
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  • Stepping Dynamics & Sequence 35. Stepping Dynamics & Sequence 3405.4724677265585
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  • Sequence Dependence 36. Sequence Dependence 3489.1495787059703
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  • Active Vs. Passive Unwinding 37. Active Vs. Passive Unwinding 3505.2651704501536
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  • Optical Trapping and Fluorescence 38. Optical Trapping and Fluoresce… 3602.0826870055912
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  • Limitations of Optical Traps 39. Limitations of Optical Traps 3615.3470586719573
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  • Fluoresence and Traps 40. Fluoresence and Traps 3676.8342394805327
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  • Fluorophore Bleaching 41. Fluorophore Bleaching 3758.7758251951864
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  • Solutions to Bleaching 42. Solutions to Bleaching 3762.7427400860624
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  • Interlacing Dual Traps 43. Interlacing Dual Traps 3764.97412971218
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  • Interlacing and Timesharing 44. Interlacing and Timesharing 3778.2385013785461
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  • Fluorescence & Trap 45. Fluorescence & Trap 3781.4616197273831
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  • Structure and Function Measurements 46. Structure and Function Measure… 3873.6923909402458
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  • UvrD Helicase 47. UvrD Helicase 3880.1386276379194
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  • UvrD Oligomeric State 48. UvrD Oligomeric State 3911.1301502228862
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  • UvrD 2B-Domain Orientation 49. UvrD 2B-Domain Orientation 3984.5180757040894
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  • Hairpin Assay 50. Hairpin Assay 4074.9409876374566
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  • Counting Proteins 51. Counting Proteins 4091.0719988986789
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  • Measuring UvrD Confirmation 52. Measuring UvrD Confirmation 4243.3283388560731
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  • Confirmation and Directionality 53. Confirmation and Directionalit… 4382.2716115261474
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  • Strand Reversal 54. Strand Reversal 4436.1340859030834
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  • Outlook 55. Outlook 4531.4862660229728
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  • Themes in Research 56. Themes in Research 4533.8984760197218
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  • Acknowledgements 57. Acknowledgements 4624.061759595641
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  • Thank You 58. Thank You 4694.5511756569849
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