BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use

By James Leary

Basic Medical Sciences and Biomedical Engineering, Purdue University, West Lafayette, IN

View Presentation (SWF)

Additional materials available (5)

Licensed under General Performance Usage.

Published on


  1. Overview – the in-vitro to ex-vivo to in-vivo paradigm
    1. In-vitro - importance of choosing suitable cell lines
    2. Ex-vivo – adding the complexity of in-vivo background while keeping the simplicity of in-vitro
    3. In-vivo - all the complexity of ex-vivo plus the “active” components of a real animal
  2. In-vivo systems are open, “active” systems with multiple layers of complexity
    1. In-vitro and ex-vivo are mostly “closed” systems, but not absolutely
    2. What is an “open” system?
    3. Attempts to isolate open systems
  3. Layers of complexity of in-vivo systems
    1. Human cells in nude mice – a mixture of in-vitro and in-vivo
    2. “Model” small animal systems
    3. Bbetter model larger animal systems
  4. Examples of the in-vitro to in-vivo experimental pathway
    1. Kopelman group – multifunctional NPs for MRI and photodynamic therapy
    2. Langer group – aptamer-targeted NPs for cancer therapy in-vivo
    3. Leary group – peptide-guided NPs to human tumors in nude mice magnetic nanoparticles as MRI contrast agents in tissue phantoms


  • Kopelman, R., Koo, Y-E, Philbert, M., Moffatc, B.A., Reddy,G.R., McConville, P., Hall, D.E., Chenevert, T.L., Bhojanie, M.S., Buck, S.M., Rehemtulla, A., Ross, B.D. Multifunctional nanoparticle platforms for in vivo MRI enhancement and photodynamic therapy of a rat brain cancer. Journal of Magnetism and Magnetic Materials 293: 404–410, 2005.
  • Farokhzad, O.C., Cheng, J., Teply, B.J., Sherifi, I., Jon, S., Kantoff, P.W., Richie, J.P., Langer, R. Targeted nanoparticle-aptamer bioconjugates for cancer chemotherapy in vivo. PNAS 103(16), 6315–6320, 2006

Cite this work

Researchers should cite this work as follows:

  • James Leary (2007), "BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use,"

    BibTex | EndNote



Biomedical Engineering Building, Room 1083