The PowerPoint file combined with the accompanying videos comprises the introductory training presentation for 3D Cell Culture Basics that make use of gels and is focused on techniques to be used for cells that form spheroid-like structures in vivo, notably a number of tumors and certain normal epithelial and neural structures.
The purpose of 3D cell culture is to place cells in an environment in which they reproduce physiologically relevant features of tissues. There is a wide range of techniques in setting up 3D cell cultures; however, fundamentally, the aim is to help cells behave as they would in the body by bringing, most often simple, modifications of the cell culture environment. The presentation covers some basic techniques for the setup of cell cultures using drip and thick embedding methods with EHS (Engelbreth-Holm-Swarm)-derived gel. Note that for each type of gel used, it is important to follow the manufacturer’s instructions and publications if available. Our presentation highlights fundamental principles of cell culture with a gel. The presentation also covers adding and removing cell culture medium in the presence of any gel, the use of serum-free medium, dislodging multicellular structures from Matrigel-based 3D culture and preparing 3D cultures for immunolabeling.
How to use this training presentation:
1. Download the PowerPoint file by clicking on the Download button found in the upper right of the screen.
2. View the PowerPoint presentation by stepping through the pages.
3. When reaching a video page, click on either the YouTube link or the download link in the next slide.
4. After watching the video, return to the PowerPoint presentation to continue the training.
A transcript for the videos can be found in the notes area for each video slide or can be downloaded.
Disclaim: The video is edited and does not reflect the actual time sequence of the procedure.
Video: Drs. Shirisha Chittiboyina and Sophie Lelièvre
3D3C Facility, Birck Nanotechnology Center, Discovery Park, Purdue University.
Cite this work
Researchers should cite this work as follows:
- Sophie A. Lelièvre; Shirisha Chittiboyina; Tim Kwok (2017), "3D Cell Culture Basics: Forming Spheroids in the Presence of Extracellular Matrix," https://nanohub.org/resources/25058.