3D CELL CULTURE
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Created: 09 Sep 2015
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3D vs 2D Cell Culture
Two dimensional (2D) cell culture techniques, in which cells are directly cultured on dishes and flasks, are common practice in the laboratory. The major drawback is, however, that the cells are not in a realistic physiological environment. In vivo, cells are in a 3-dimensional (3D) environment with major physical and chemical contacts such as interactions with surrounding cells, and with the extracellular matrix.
Cell and tissue cultures that recapitulate specific cell arrangements in 3D permit the identification of pathways of normal tissue function as well as the exquisite study of factors of disease onset and progression under physiological conditions compared to standard monolayered (2D) culture. They also permit the development of tools and methods that measure biological features and drugs that target the cellular/tissue disorder under conditions that are closer to in vivo.
The use of classical 3D cell culture and engineered organ-on-a-chip systems requires training and a good understanding of what a tissue model is about. Having a 3D cell culture facility on site enables scientists to use models for the period of time needed (without the need to set the logistics for maintenance of 3D cell culture supplies), to perform 3D culture in a sheltered environment, to receive appropriate training in 3D cell culture and to get help to develop or implement a 3D cell culture model.
The 3D3C Facility in a nutshell is here..
1. To provide support for temporary or long-term experiments using 3D cell culture systems without the need to set up the 3D cell culture system within the laboratory of the PI
2. To provide training for any laboratory member in the art of 3D cell culture
3. To help with the design of new cell culture models for specific applications or types of diseases
4. To nurture equipment and methods for analysis of tissues and cells from cultures and from in vivo collection.