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Resource/NewsletterV3
Volume 3, April 2016
Newsletter
3D at Purdue
3D in Focus
3D in Publications
3D in Meetings
3D at Purdue
Bumsoo Han, Purdue University Professor of mechanical engineering, has been working on developing a chip that stimulates the tumor "microenvironment". One of his goals is to use such system to test the effectiveness of nanoparticles and drugs that target cancers. His work makes use of 3D bioprinting to create the tumor-microenvironment-on-chip (T-MOC) device with mimicry of aspects of the complex environment around tumors necessary to assess movements of particles out of blood vessels and within the tumor tissue. The idea is to use this system to gather information about the ways by which nanoparticles move through the microenvironment. The T-MOC chip is about 4.5 centimeters (1.8 inches) square. It consists of three-dimensional microfluidic channels in which tumor cells and endothelial cells are embedded within extracellular matrix and under perfusion of interstitial fluid regulated by pressure reservoirs.
In theory, to target the tumor tissue effectively without harming the rest of the body, agent delivery particles can be used that are small enough to pass through the pores located in blood vessels surrounding tumors but also large enough not to pass though the pores of vessels present in healthy tissue. The endothelial cells in the normal blood vessels are well organized and have only small pores in the tight junctions that connect them. However, the endothelial cells in blood vessels around tumors are not well-organized. Notably, larger pores than normal are present. Logically, once injected in the blood stream agent delivery particles of well-chosen size should be capable of selectively moving out of the vessels only in the tumor tissue. However, the pressure of "interstitial fluid" inside tumors is greater than that of the surrounding healthy tissue. The greater pressure inside the tumor may push out most drug-delivery systems and imaging agents, leaving only a small percentage of them inside the tumor. This is why there is a need for a better understanding of movements of agent delivery particles in the tumor surroundings.
Professor Han’s research group constructed the T-MOC chip (see Figure) by embedding human breast cancer and endothelial cells in the extracellular matrix, a spongy, scaffold-like material made of collagen I, as a main component, located in a compartment called the interstitium in living tissues. The transport of nanoparticles and its variation have been studied by the Han’s team with respect to tumor microenvironmental parameters such as cut-off pore size, interstitial fluid pressure, and tumor tissue microstructure. Findings confirm that nanoparticles should be designed by taking into account their dynamic interactions with the tumor microenvironment.
This work was highlighted in Purdue Today (by Emil Venere March 1 2016) and part of the results have been published in the Journal of Control Release*.
* Kwak B, Ozcelikkale A, Shin CS, Park K, Han B Simulation of complex transport of nanoparticles around a tumor using tumor-microenvironment-on-chip Journal of Controlled Release 194 (2014) 157–167
Drawing of the T-MOC (courtesy of Dr. Bumsoo Han)
3D in Focus
In this newsletter editorial, we chose to highlight work done to study how three-dimensional (3D) microenvironments may increase the reprogramming efficacy of somatic cells into induced pluripotent stem cells (iPSCs).
Caiazzo M, Okawa Y, Ranga A, Piersigilli A, Tabata Y, Lutolf MP Defined three-dimensional microenvironments boost induction of pluripotency. Nat Mater. 2016 Mar; 15(3):344-52.
Indeed standard 2D cell culture systems have had only limited success in terms of iPSC yield. The iPSCs are reprogrammed somatic cells prepared from a variety of tissue sources and genetic background. These cells can proliferate without limit and also maintain the potential to generate derivatives of all three embryonic germ layers (ectoderm, endoderm, or mesoderm), as such being the potential sources for different cell types like neurons, cardiomyocytes and hepatocytes. Therefore, iPSCs can be a powerful tool for regenerative medicine, disease modeling, drug screening, and precision medicine. Improving the yield of iPSCs will be greatly necessary and important in its future application in disease modeling and therapy.
In the experiments by Caiazzo et al., the enhanced power of the 3D microenvironment over standard 2D cell culture on the induction efficacy of pluripotency was first confirmed by embedding mouse embryonic stem cells in poly(ethyleneglycol) (PEG)-based hydrogels. In the subsequent study on iPSCs from somatic origin, the authors engineered a mouse system expressing the four Yamanaka factors (Oct4, Sox2, Klf4 and c-Myc) that are known to be essential for the induction of pluripotency in somatic cells. Achievement of pluripotency was monitored by an Oct4–GFP (green fluorescence protein) reporter system. Mouse tail-tip fibroblasts were encapsulated in the PEG-based hydrogels and the reprogramming efficacy was subsequently assessed based on the number of Oct4–GFP-positive iPSC colonies forming in the gels. The authors proposed that the 3D microenvironment keeps cells in an active proliferation state throughout the entire reprogramming process and that the production of iPSCs might be faster under 3D culture conditions.
Following the use of a 3D high-throughput screening approach to simultaneously probe 128 unique microenvironmental conditions, the authors concluded that the use of PEG-hydrogels with stiffness between 300 and 600 Pa, the enrichment of the microenvironment with laminin or EpCAM in the hydrogels, and the activation of the Wnt pathway, all led to marked improvements in reprogramming efficacy. Importantly, the physical confinement of cells imposed by the 3D microenvironment increased the reprogramming efficacy (measured by the number of colonies formed) of both mouse and human iPSCs by more than two-fold. Experiments also showed that early steps during reprogramming in 3D cell culture that lead to the formation of colonies, may cause both chromatin remodeling and accelerated mesenchymal-to-epithelial transition, two key events for the initiation of iPSC generation. Moreover, the increase in reprogramming efficacy comparable to that of PEG was also demonstrated in cells embedded in Matrigel.
Overall, the study demonstrates that the modulation of the microenvironment in 3D cell culture is crucial to improve reprogramming effectiveness to a level that may not be achievable with 2D cell culture.
3D in Publications
Complete List -- 3D culture related articles on PubMed (last 2 months)
Reviews
Scaffold free/Scaffold
Organ/Tissue/Cell
Others
3D in Publications
Complete List
Akasov, R., Zaytseva-Zotova, D., Burov, S., Leko, M., Dontenwill, M., Chiper, M., Vandamme, T., and Markvicheva, E. (2016). Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro. Int J Pharm.
Albritton, J. L., Roybal, J. D., Paulsen, S. J., Calafat, N., Flores-Zaher, J. A., Farach-Carson, M. C., Gibbons, D. L., and Miller, J. S. (2016). Ultrahigh-throughput Generation and Characterization of Cellular Aggregates in Laser-ablated Microwells of Poly(dimethylsiloxane). RSC Adv 6, 8980-8991.
Alessandri, K., Feyeux, M., Gurchenkov, B., Delgado, C., Trushko, A., Krause, K. H., Vignjevic, D., Nassoy, P., and Roux, A. (2016). A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC). Lab Chip.
Almela, T., Brook, I. M., and Moharamzadeh, K. (2016). Development of three-dimensional tissue engineered bone-oral mucosal composite models. J Mater Sci Mater Med 27, 65.
Alonso-Nocelo, M., Abuin, C., Lopez-Lopez, R., and de la Fuente, M. (2016). Development and characterization of a three-dimensional co-culture model of tumor T cell infiltration. Biofabrication 8, 025002.
Arevalos, C. A., Berg, J. M., Nguyen, J. M., Godfrey, E. L., Iriondo, C., and Grande-Allen, K. J. (2016). Valve Interstitial Cells Act in a Pericyte Manner Promoting Angiogensis and Invasion by Valve Endothelial Cells. Ann Biomed Eng.
Arnal-Pastor, M., Comin-Cebrian, S., Martinez-Ramos, C., Monleon Pradas, M., and Valles-Lluch, A. (2016). Hydrophilic surface modification of acrylate-based biomaterials. J Biomater Appl 30, 1429-1441.
Arpin, C. C., Mac, S., Jiang, Y., Cheng, H., Grimard, M., Page, B. D., Kamocka, M. M., Haftchenary, S., Su, H., Ball, D. P., et al. (2016). Applying Small Molecule Signal Transducer and Activator of Trancription-3 (STAT3) Protein Inhibitors as Pancreatic Cancer Therapeutics. Mol Cancer Ther.
Arslan, E., Guler, M. O., and Tekinay, A. B. (2016). Glycosaminoglycan-Mimetic Signals Direct the Osteo/Chondrogenic Differentiation of Mesenchymal Stem Cells in a Three-Dimensional Peptide Nanofiber Extracellular Matrix Mimetic Environment. Biomacromolecules 17, 1280-1291.
Asthana, A., and Kisaalita, W. S. (2016). Molecular basis for cytokine biomarkers of complex 3D microtissue physiology in vitro. Drug Discov Today.
Barata, D., van Blitterswijk, C., and Habibovic, P. (2016). High-throughput screening approaches and combinatorial development of biomaterials using microfluidics. Acta Biomater 34, 1-20.
Bellotti, C., Duchi, S., Bevilacqua, A., Lucarelli, E., and Piccinini, F. (2016). Long term morphological characterization of mesenchymal stromal cells 3D spheroids built with a rapid method based on entry-level equipment. Cytotechnology.
Bilgin, C. C., Fontenay, G., Cheng, Q., Chang, H., Han, J., and Parvin, B. (2016). BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models. PLoS One 11, e0148379.
Bono, N., Pezzoli, D., Levesque, L., Loy, C., Candiani, G., Fiore, G. B., and Mantovani, D. (2016). Unraveling the role of mechanical stimulation on smooth muscle cells: A comparative study between 2D and 3D models. Biotechnol Bioeng.
Boyce, M. W., Kenney, R. M., Truong, A. S., and Lockett, M. R. (2016). Quantifying oxygen in paper-based cell cultures with luminescent thin film sensors. Anal Bioanal Chem 408, 2985-2992.
Calabrese, R., Raia, N., Huang, W., Ghezzi, C. E., Simon, M., Staii, C., Weiss, A. S., and Kaplan, D. L. (2016). Silk-ionomer and silk-tropoelastin hydrogels as charged three-dimensional culture platforms for the regulation of hMSC response. J Tissue Eng Regen Med.
Cardoso, T. C., Sakamoto, S. S., Stockmann, D., Souza, T. F., Ferreira, H. L., Gameiro, R., Vieira, F. V., Louzada, M. J., Andrade, A. L., and Flores, E. F. (2016). A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators. Vet Comp Oncol.
Casey, A., Gargotti, M., Bonnier, F., and Byrne, H. J. (2016). Chemotherapeutic efficiency of drugs in vitro: Comparison of doxorubicin exposure in 3D and 2D culture matrices. Toxicol In Vitro 33, 99-104.
Cesarz, Z., Funnell, J. L., Guan, J., and Tamama, K. (2016). Soft Elasticity-Associated Signaling and Bone Morphogenic Protein 2 are Key Regulators of Mesenchymal Stem Cell Spheroidal Aggregates. Stem Cells Dev.
Chamorro, C. I., Zeiai, S., Reinfeldt Engberg, G., and Fossum, M. (2016). Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair. J Vis Exp.
Chen, W., Ma, J., Zhu, L., Morsi, Y., Ei-Hamshary, H., Al-Deyab, S. S., and Mo, X. (2016). Superelastic, superabsorbent and 3D nanofiber-assembled scaffold for tissue engineering. Colloids Surf B Biointerfaces 142, 165-172.
Chi, C. W., Ahmed, A. R., Dereli-Korkut, Z., and Wang, S. (2016). Microfluidic cell chips for high throughput drug screening. Bioanalysis, 0.
Choi, C. H., Wang, H., Lee, H., Kim, J. H., Zhang, L., Mao, A., Mooney, D. J., and Weitz, D. A. (2016). One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell. Lab Chip.
Christoffersson, J., Bergstrom, G., Schwanke, K., Kempf, H., Zweigerdt, R., and Mandenius, C. F. (2016). A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies. Methods Mol Biol.
Dai, G., Wan, W., Zhao, Y., Wang, Z., Li, W., Shi, P., and Shen, Y. (2016). Controllable 3D alginate hydrogel patterning via visible-light induced electrodeposition. Biofabrication 8, 025004.
Dehdilani, N., Shamsasenjan, K., Movassaghpour, A., Akbarzadehlaleh, P., Amoughli Tabrizi, B., Parsa, H., and Sabagi, F. (2016). Improved Survival and Hematopoietic Differentiation of Murine Embryonic Stem Cells on Electrospun Polycaprolactone Nanofiber. Cell J 17, 629-638.
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Duan, B., Yin, Z., Hockaday Kang, L., Magin, R. L., and Butcher, J. T. (2016). Active tissue stiffness modulation controls valve interstitial cell phenotype and osteogenic potential in 3D culture. Acta Biomater.
Duarte Campos, D. F., Blaeser, A., Buellesbach, K., Sen, K. S., Xun, W., Tillmann, W., and Fischer, H. (2016). Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering. Adv Healthc Mater.
Emura, M., and Aufderheide, M. (2016). Challenge for 3D culture technology: Application in carcinogenesis studies with human airway epithelial cells. Exp Toxicol Pathol.
Faghihi, F., Mirzaei, E., Ai, J., Lotfi, A., Sayahpour, F. A., Barough, S. E., and Joghataei, M. T. (2016). Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems. Mol Neurobiol 53, 1862-1872.
Ferraz, E. R., Rainho, C. R., Fernandes, A. S., and Felzenszwalb, I. (2016). Differential Toxicity of an Organic PM Extract to Human Lung Cells Cultured in Three Dimensions (3D) and Monolayers. J Toxicol Environ Health A, 1-11.
Fischer, K. M., Morgan, K. Y., Hearon, K., Sklaviadis, D., Tochka, Z. L., Fenton, O. S., Anderson, D. G., Langer, R., and Freed, L. E. (2016). Poly(Limonene Thioether) Scaffold for Tissue Engineering. Adv Healthc Mater.
Follin, B., Juhl, M., Cohen, S., Perdersen, A. E., Kastrup, J., and Ekblond, A. (2016). Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture. Tissue Eng Part B Rev.
Gerges, I., Tamplenizza, M., Rossi, E., Tocchio, A., Martello, F., Recordati, C., Kumar, D., Forsyth, N. R., Liu, Y., and Lenardi, C. (2016). A Tailor-Made Synthetic Polymer for Cell Encapsulation: Design Rationale, Synthesis, Chemical-Physics and Biological Characterizations. Macromol Biosci.
Gilmour, A. D., Woolley, A. J., Poole-Warren, L. A., Thomson, C. E., and Green, R. A. (2016). A critical review of cell culture strategies for modelling intracortical brain implant material reactions. Biomaterials 91, 23-43.
Gonzalez, S., Mei, H., Nakatsu, M. N., Baclagon, E. R., and Deng, S. X. (2016). A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16, 358-364.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Han, S., Wang, B., Li, X., Xiao, Z., Han, J., Zhao, Y., Fang, Y., Yin, Y., Chen, B., and Dai, J. (2016). Bone marrow-derived mesenchymal stem cells in three-dimensional culture promote neuronal regeneration by neurotrophic protection and immunomodulation. J Biomed Mater Res A.
Hara, J., Tottori, J., Anders, M., Dadhwal, S., Asuri, P., and Mobed-Miremadi, M. (2016). Trehalose effectiveness as a cryoprotectant in 2D and 3D cell cultures of human embryonic kidney cells. Artif Cells Nanomed Biotechnol, 1-8.
Harris, S., Wegner, S., Hong, S. W., and Faustman, E. M. (2016). Phthalate metabolism and kinetics in an in vitro model of testis development. Toxicol In Vitro 32, 123-131.
He, P., Fu, J., and Wang, D. A. (2016). Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform. Acta Biomater.
Hof, A., Raschke, S., Baier, K., Nehrenheim, L., Selig, J. I., Schomaker, M., Lichtenberg, A., Meyer, H., and Akhyari, P. (2016). Challenges in developing a reseeded, tissue-engineered aortic valve prosthesis. Eur J Cardiothorac Surg.
Huang, Z., Zhen, Y., Yin, W., Ma, Z., and Zhang, L. (2016). Shh promotes sweat gland cell maturation in three-dimensional culture. Cell Tissue Bank.
Ishii, T., Saito, H., Komizu, Y., Tomoshige, R., and Matsushita, T. (2016). Effects of macroporous hydroxyapatite carriers on the growth and function of human hepatoblasts derived from fetal hepatocytes. J Biosci Bioeng.
Itou, J., Tanaka, S., Li, W., Matsumoto, Y., Sato, F., and Toi, M. (2015). Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells. Data Brief 5, 429-433.
Jobe, N. P., Rosel, D., Dvorankova, B., Kodet, O., Lacina, L., Mateu, R., Smetana, K., and Brabek, J. (2016). Simultaneous blocking of IL-6 and IL-8 is sufficient to fully inhibit CAF-induced human melanoma cell invasiveness. Histochem Cell Biol.
Kang, L. H., Armstrong, P. A., Lee, L. J., Duan, B., Kang, K. H., and Butcher, J. T. (2016). Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels. Ann Biomed Eng.
Kato, T., Oka, K., Nakamura, T., and Ito, A. (2016). Decreased expression of Met during differentiation in rat lung. Eur J Histochem 60, 2575.
Katt, M. E., Placone, A. L., Wong, A. D., Xu, Z. S., and Searson, P. C. (2016). In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform. Front Bioeng Biotechnol 4, 12.
Katti, K. S., Molla, S., Karandish, F., Haldar, M. K., Mallik, S., and Katti, D. R. (2016). Sequential culture on biomimetic nanoclay scaffolds forms three dimensional tumoroids. J Biomed Mater Res A.
Keely, P., and Nain, A. (2015). Capturing relevant extracellular matrices for investigating cell migration. F1000Res 4.
Kim, J., and Tanner, K. (2016). Three-Dimensional Patterning of the ECM Microenvironment Using Magnetic Nanoparticle Self Assembly. Curr Protoc Cell Biol 70, 25.23.21-25.23.14.
Kingsley, D. M., Dias, A. D., and Corr, D. T. (2016). Microcapsules and 3D customizable shelled microenvironments from laser direct-written microbeads. Biotechnol Bioeng.
Klotz, B. J., Gawlitta, D., Rosenberg, A. J., Malda, J., and Melchels, F. P. (2016). Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair. Trends Biotechnol.
Ko, K. R., and Frampton, J. P. (2016). Developments in 3D neural cell culture models: the future of neurotherapeutics testing? Expert Rev Neurother, 1-3.
Koeck, S., Zwierzina, M., Huber, J. M., Bitsche, M., Lorenz, E., Gamerith, G., Dudas, J., Kelm, J. M., Zwierzina, H., and Amann, A. (2016). Infiltration of lymphocyte subpopulations into cancer microtissues as a tool for the exploration of immunomodulatory agents and biomarkers. Immunobiology 221, 604-617.
Koutsopoulos, S. (2016). Self-assembling peptide nanofiber hydrogels in tissue engineering and regenerative medicine: Progress, design guidelines, and applications. J Biomed Mater Res A 104, 1002-1016.
Labour, M. N., Vigier, S., Lerner, D., Marcilhac, A., and Belamie, E. (2016). 3D compartmented model to study the neurite-related toxicity of Abeta aggregates included in collagen gels of adaptable porosity. Acta Biomater.
Lambros, M. P., DeSalvo, M. K., Mulamalla, H. C., Moreno, J., and Kondapalli, L. (2016). Genome wide expression after different doses of irradiation of a three-dimensional (3D) model of oral mucosal. Genom Data 7, 137-139.
Langan, L. M., Dodd, N. J., Owen, S. F., Purcell, W. M., Jackson, S. K., and Jha, A. N. (2016). Direct Measurements of Oxygen Gradients in Spheroid Culture System Using Electron Parametric Resonance Oximetry. PLoS One 11, e0149492.
Lapa, G. B., Tsunoda, T., Shirasawa, S., Baryshnikova, M. A., Evseev, G. G., Afanasyeva, D. A., and Chigorina, E. A. (2016). Synthesis of New Congeners of 1-methyl-3-aminoisoquinolines, Evaluation of Their Cytotoxic Activity, In Silico and In Vitro Study of Their Molecular Targets as PDE4B. Chem Biol Drug Des 87, 575-582.
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Lee, J. H., Lee, H. H., Kim, K. N., and Kim, K. M. (2016). Cytotoxicity and anti-inflammatory effects of zinc ions and eugenol during setting of ZOE in immortalized human oral keratinocytes grown as three-dimensional spheroids. Dent Mater.
Lei, Y., and Ferdous, Z. (2016). Design Considerations and Challenges for Mechanical Stretch Bioreactors in Tissue Engineering. Biotechnol Prog.
Li, C. W., Pan, W. T., Ju, J. C., and Wang, G. J. (2016a). An endothelial cultured condition medium embedded porous PLGA scaffold for the enhancement of mouse embryonic stem cell differentiation. Biomed Mater 11, 025015.
Li, Y., Huang, G., Li, M., Wang, L., Elson, E. L., Jian Lu, T., Genin, G. M., and Xu, F. (2016b). An approach to quantifying 3D responses of cells to extreme strain. Sci Rep 6, 19550.
Lin, B., Miao, Y., Wang, J., Fan, Z., Du, L., Su, Y., Liu, B., Hu, Z., and Xing, M. (2016). Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration. ACS Appl Mater Interfaces 8, 5906-5916.
Liu, H., Liu, J., Qi, C., Fang, Y., Zhang, L., Zhuo, R., and Jiang, X. (2016a). Thermosensitive injectable in-situ forming carboxymethyl chitin hydrogel for three-dimensional cell culture. Acta Biomater 35, 228-237.
Liu, Z., Takeuchi, M., Nakajima, M., Hasegawa, Y., Huang, Q., and Fukuda, T. (2016b). Shape-controlled high cell-density microcapsules by electrodeposition. Acta Biomater.
Lo, Y. P., Liu, Y. S., Rimando, M. G., Ho, J. H., Lin, K. H., and Lee, O. K. (2016). Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells. Sci Rep 6, 21253.
Loessner, D., Meinert, C., Kaemmerer, E., Martine, L. C., Yue, K., Levett, P. A., Klein, T. J., Melchels, F. P., Khademhosseini, A., and Hutmacher, D. W. (2016). Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. Nat Protoc 11, 727-746.
Lolli, A., Narcisi, R., Lambertini, E., Penolazzi, L., Angelozzi, M., Kops, N., Gasparini, S., van Osch, G. J., and Piva, R. (2016). Silencing of anti-chondrogenic microRNA-221 in human mesenchymal stem cells promotes cartilage repair in vivo. Stem Cells.
Lu, L., and Unsworth, L. D. (2016). pH-Triggered Release of Hydrophobic Molecules from Self-Assembling Hybrid Nanoscaffolds. Biomacromolecules.
Luckert, C., Schulz, C., Lehmann, N., Thomas, M., Hofmann, U., Hammad, S., Hengstler, J. G., Braeuning, A., Lampen, A., and Hessel, S. (2016). Comparative analysis of 3D culture methods on human HepG2 cells. Arch Toxicol.
McConkey, C. A., Delorme-Axford, E., Nickerson, C. A., Kim, K. S., Sadovsky, Y., Boyle, J. P., and Coyne, C. B. (2016). A three-dimensional culture system recapitulates placental syncytiotrophoblast development and microbial resistance. Sci Adv 2, e1501462.
Medda, X., Mertens, L., Versweyveld, S., Diels, A., Barnham, L., Bretteville, A., Buist, A., Verheyen, A., Royaux, I., Ebneth, A., and Cabrera-Socorro, A. (2016). Development of a Scalable, High-Throughput-Compatible Assay to Detect Tau Aggregates Using iPSC-Derived Cortical Neurons Maintained in a Three-Dimensional Culture Format. J Biomol Screen.
Meenach, S. A., Tsoras, A. N., McGarry, R. C., Mansour, H. M., Hilt, J. Z., and Anderson, K. W. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. Int J Oncol 48, 1701-1709.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Nemati, S., Abbasalizadeh, S., and Baharvand, H. (2016). Scalable Expansion of Human Pluripotent Stem Cell-Derived Neural Progenitors in Stirred Suspension Bioreactor Under Xeno-free Condition. Methods Mol Biol.
Ning, L., Xu, Y., Chen, X., and Schreyer, D. J. (2016). Influence of mechanical properties of alginate-based substrates on the performance of Schwann cells in culture. J Biomater Sci Polym Ed, 1-18.
Niu, C., Chauhan, U., Gargus, M., and Shaker, A. (2016). Generation and Characterization of an Immortalized Human Esophageal Myofibroblast Line. PLoS One 11, e0153185.
Nowotny, J., Aibibu, D., Farack, J., Nimtschke, U., Hild, M., Gelinsky, M., Kasten, P., and Cherif, C. (2016). Novel fiber-based pure chitosan scaffold for tendon augmentation: biomechanical and cell biological evaluation. J Biomater Sci Polym Ed, 1-20.
O'Leary, C., Cavanagh, B., Unger, R. E., Kirkpatrick, C. J., O'Dea, S., O'Brien, F. J., and Cryan, S. A. (2016). The development of a tissue-engineered tracheobronchial epithelial model using a bilayered collagen-hyaluronate scaffold. Biomaterials 85, 111-127.
Oberbauer, E., Steffenhagen, C., Feichtinger, G., Hildner, F., Hacobian, A., Danzer, M., Gabriel, C., Redl, H., and Wolbank, S. (2016). A luciferase-based quick potency assay to predict chondrogenic differentiation. Tissue Eng Part C Methods.
Oldenburg, A. L., Yu, X., Gilliss, T., Alabi, O., Taylor, R. M., 2nd, and Troester, M. A. (2015). Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy. Optica 2, 877-885.
Otani, T., Marchetto, M. C., Gage, F. H., Simons, B. D., and Livesey, F. J. (2016). 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. Cell Stem Cell 18, 467-480.
Parvizi, M., Bolhuis-Versteeg, L. A., Poot, A. A., and Harmsen, M. C. (2016). Efficient generation of smooth muscle cells from adipose-derived stromal cells by 3D mechanical stimulation can substitute the use of growth factors in vascular tissue engineering. Biotechnol J.
Pascu, E. I., Cahill, P. A., Stokes, J., and McGuinness, G. B. (2016). Towards functional 3D-stacked electrospun composite scaffolds of PHBV, silk fibroin and nanohydroxyapatite: Mechanical properties and surface osteogenic differentiation. J Biomater Appl 30, 1334-1349.
Patel, N. M., Yazdi, I. K., Tasciotti, E., and Birla, R. K. (2016). Optimizing cell seeding and retention in a three-dimensional bioengineered cardiac ventricle: The two-stage cellularization model. Biotechnol Bioeng.
Pati, F., Gantelius, J., and Svahn, H. A. (2016). 3D Bioprinting of Tissue/Organ Models. Angew Chem Int Ed Engl.
Patra, B., Peng, C. C., Liao, W. H., Lee, C. H., and Tung, Y. C. (2016). Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device. Sci Rep 6, 21061.
Paulose, T., Montevil, M., Speroni, L., Cerruti, F., Sonnenschein, C., and Soto, A. M. (2016). SAMA: A Method for 3D Morphological Analysis. PLoS One 11, e0153022.
Pellman, J., Zhang, J., and Sheikh, F. (2016). Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems. J Mol Cell Cardiol.
Penolazzi, L., Lolli, A., Sardelli, L., Angelozzi, M., Lambertini, E., Trombelli, L., Ciarpella, F., RenataVecchiatini, and Piva, R. (2016). Establishment of a 3D-dynamic osteoblasts-osteoclasts co-culture model to simulate the jawbone microenvironment in vitro. Life Sci.
Petropolis, D. B., Faust, D. M., Tolle, M., Riviere, L., Valentin, T., Neuveut, C., Hernandez-Cuevas, N., Dufour, A., Olivo-Marin, J. C., and Guillen, N. (2016). Human Liver Infection in a Dish: Easy-To-Build 3D Liver Models for Studying Microbial Infection. PLoS One 11, e0148667.
Pinnock, C. B., Meier, E. M., Joshi, N. N., Wu, B., and Lam, M. T. (2016). Customizable engineered blood vessels using 3D printed inserts. Methods 99, 20-27.
Pouliot, R. A., Link, P. A., Mikhaiel, N. S., Schneck, M. B., Valentine, M. S., Kamga Gninzeko, F. J., Herbert, J. A., Sakagami, M., and Heise, R. L. (2016). Development and characterization of a naturally derived lung extracellular matrix hydrogel. J Biomed Mater Res A.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Raghothaman, D., Leong, M. F., Lim, T. C., Wan, A. C., Ser, Z., Lee, E. H., and Yang, Z. (2016). Cell type dependent morphological adaptation in polyelectrolyte hydrogels governs chondrogenic fate. Biomed Mater 11, 025013.
Ramadan, Q., and Ting, F. C. (2016). In vitro micro-physiological immune-competent model of the human skin. Lab Chip.
Ramakrishnan, V. M., Tien, K. T., McKinley, T. R., Bocard, B. R., McCurry, T. M., Williams, S. K., Hoying, J. B., and Boyd, N. L. (2016). Wnt5a Regulates the Assembly of Human Adipose Derived Stromal Vascular Fraction-Derived Microvasculatures. PLoS One 11, e0151402.
Recha-Sancho, L., and Semino, C. E. (2016). Heparin based self-assembling peptide scaffold reestablish chondrogenic phenotype of expanded de-differentiated human chondrocytes. J Biomed Mater Res A.
Relier, S., Yazdani, L., Ayad, O., Choquet, A., Bourgaux, J. F., Prudhomme, M., Pannequin, J., Macari, F., and David, A. (2016). Antibiotics inhibit sphere-forming ability in suspension culture. Cancer Cell Int 16, 6.
Robinson, S. T., Douglas, A. M., Chadid, T., Kuo, K., Rajabalan, A., Li, H., Copland, I. B., Barker, T. H., Galipeau, J., and Brewster, L. P. (2016). A Novel Platelet Lysate Hydrogel for Endothelial Cell and Mesenchymal Stem Cell-Directed Neovascularization. Acta Biomater.
Ryan, S. L., Baird, A. M., Vaz, G., Urquhart, A. J., Senge, M., Richard, D. J., O'Byrne, K. J., and Davies, A. M. (2016). Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture. Assay Drug Dev Technol 14, 19-28.
Sarkar, J., and Kumar, A. (2016). Thermo-responsive polymer aided spheroid culture in cryogel based platform for high throughput drug screening. Analyst.
Sato, K., Choyke, P. L., and Hisataka, K. (2016). Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique. J Vis Exp.
Schmidt, M., Scholz, C. J., Polednik, C., and Roller, J. (2016). Spheroid-based 3-dimensional culture models: Gene expression and functionality in head and neck cancer. Oncol Rep 35, 2431-2440.
Schuurman, W., Harimulyo, E. B., Gawlitta, D., Woodfield, T. B., Dhert, W. J., van Weeren, P. R., and Malda, J. (2016). Three-dimensional assembly of tissue-engineered cartilage constructs results in cartilaginous tissue formation without retainment of zonal characteristics. J Tissue Eng Regen Med 10, 315-324.
Scioli, M. G., Bielli, A., Gentile, P., Cervelli, V., and Orlandi, A. (2016). Combined treatment with platelet-rich plasma and insulin favours chondrogenic and osteogenic differentiation of human adipose-derived stem cells in three-dimensional collagen scaffolds. J Tissue Eng Regen Med.
Shalaly, N. D., Ria, M., Johansson, U., Avall, K., Berggren, P. O., and Hedhammar, M. (2016). Silk matrices promote formation of insulin-secreting islet-like clusters. Biomaterials 90, 50-61.
Simao, D., Arez, F., Terasso, A. P., Pinto, C., Sousa, M. F., Brito, C., and Alves, P. M. (2016). Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells. Methods Mol Biol.
Sims, L. B., Curtis, L. T., Frieboes, H. B., and Steinbach-Rankins, J. M. (2016). Enhanced uptake and transport of PLGA-modified nanoparticles in cervical cancer. J Nanobiotechnology 14, 33.
Smith Callahan, L. A. (2016). The concentration game: differential effects of bioactive signaling in 2D and 3D culture. Neural Regen Res 11, 66-68.
Sokol, E. S., Miller, D. H., Breggia, A., Spencer, K. C., Arendt, L. M., and Gupta, P. B. (2016). Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Breast Cancer Res 18, 19.
Soon, C. F., Thong, K. T., Tee, K. S., Ismail, A. B., Denyer, M., Ahmad, M. K., Kong, Y. H., Vyomesh, P., and Cheong, S. C. (2016). A scaffoldless technique for self-generation of three-dimensional keratinospheroids on liquid crystal surfaces. Biotech Histochem, 1-13.
Speroni, L., Sweeney, M. F., Sonnenschein, C., and Soto, A. M. (2016). A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium. J Vis Exp.
Suntornnond, R., An, J., Tijore, A., Leong, K. F., Chua, C. K., and Tan, L. P. (2016). A Solvent-Free Surface Suspension Melt Technique for Making Biodegradable PCL Membrane Scaffolds for Tissue Engineering Applications. Molecules 21.
Tan, Y. J., Yeong, W. Y., Tan, X., An, J., Chian, K. S., and Leong, K. F. (2016). Characterization, mechanical behavior and in vitro evaluation of a melt-drawn scaffold for esophageal tissue engineering. J Mech Behav Biomed Mater 57, 246-259.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Tonello, S., Moore, M. C., Sharma, B., Dobson, J., and McFetridge, P. S. (2016). Controlled release of a heterogeneous human placental matrix from PLGA microparticles to modulate angiogenesis. Drug Deliv Transl Res 6, 174-183.
Tutak, W., Jyotsnendu, G., Bajcsy, P., and Simon, C. G., Jr. (2016). Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. J Biomed Mater Res B Appl Biomater.
Ugbode, C. I., Hirst, W. D., and Rattray, M. (2016). Astrocytes Grown in Alvetex Three Dimensional Scaffolds Retain a Non-reactive Phenotype. Neurochem Res.
Vantangoli, M. M., Wilson, S., Madnick, S. J., Huse, S. M., and Boekelheide, K. (2016). Morphologic effects of estrogen stimulation on 3D MCF-7 microtissues. Toxicol Lett 248, 1-8.
Viguier, A., Boyer, C., Chassenieux, C., Benyahia, L., Guicheux, J., Weiss, P., Rethore, G., and Nicolai, T. (2016). Interpenetrated Si-HPMC/alginate hydrogels as a potential scaffold for human tissue regeneration. J Mater Sci Mater Med 27, 99.
Waisbourd-Zinman, O., Koh, H., Tsai, S., Lavrut, P. M., Dang, C., Zhao, X., Pack, M., Cave, J., Hawes, M., Koo, K. A., et al. (2016). The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis via decreased glutathione and SOX17. Hepatology.
Wang, H., Wu, G., Zhang, J., Zhou, K., Yin, B., Su, X., Qiu, G., Yang, G., Zhang, X., Zhou, G., and Wu, Z. (2016a). Osteogenic effect of controlled released rhBMP-2 in 3D printed porous hydroxyapatite scaffold. Colloids Surf B Biointerfaces 141, 491-498.
Wang, J., Chen, F., Liu, L., Qi, C., Wang, B., Yan, X., Huang, C., Hou, W., Zhang, M. Q., Chen, Y., and Du, Y. (2016b). Engineering EMT using 3D micro-scaffold to promote hepatic functions for drug hepatotoxicity evaluation. Biomaterials 91, 11-22.
Wang, J. Z., Zhu, Y. X., Ma, H. C., Chen, S. N., Chao, J. Y., Ruan, W. D., Wang, D., Du, F. G., and Meng, Y. Z. (2016c). Developing multi-cellular tumor spheroid model (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. Mater Sci Eng C Mater Biol Appl 62, 215-225.
Wang, Q. A., and Scherer, P. E. (2016). Human Beige Adipocytes: Epiphenomenon or Drivers of Metabolic Improvements? Trends Endocrinol Metab.
Weber, L., Langer, M., Tavella, S., Ruggiu, A., and Peyrin, F. (2016). Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells. Phys Med Biol 61, N215-n231.
Weigand, A., Boos, A. M., Tasbihi, K., Beier, J. P., Dalton, P. D., Schrauder, M., Horch, R. E., Beckmann, M. W., Strissel, P. L., and Strick, R. (2016). Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells. Breast Cancer Res 18, 32.
Wu, Y., Sun, J., George, J., Liu, Q., Ye, H., Ge, D., Liu, Y., Zhang, Y., Cui, Z., and Li, Z. (2016). Study of Neuroprotective Function of Ginkgo biloba Extract (EGb761) Derived-flavonoid Monomers Using a Three-dimensional Stem Cell-derived Neural Model. Biotechnol Prog.
Xie, Q., Xie, J., Zhong, J., Cun, X., Lin, S., Lin, Y., and Cai, X. (2016). Hypoxia enhances angiogenesis in an adipose-derived stromal cell/endothelial cell co-culture 3D gel model. Cell Prolif.
Xu, H., Liu, W., Zhang, X. Z., Hou, L., Lu, Y. J., Chen, P. P., Zhang, C., Feng, D., Kong, L., and Wang, X. L. (2016a). [Development of three-dimensional breast cancer cell culture drug resistance model]. Sheng Li Xue Bao 68, 179-184.
Xu, Y., Shi, T., Xu, A., and Zhang, L. (2016b). 3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney. J Cell Mol Med.
Yassin, M. A., Leknes, K. N., Sun, Y., Lie, S. A., Finne-Wistrand, A., and Mustafa, K. (2016). Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation. J Biomed Mater Res A.
Ye, J., Wang, J., Zhu, Y., Wei, Q., Wang, X., Yang, J., Tang, S., Liu, H., Fan, J., Zhang, F., et al. (2016). A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells. Biomed Mater 11, 025021.
Yoon, H. I., Yhee, J. Y., Na, J. H., Lee, S., Lee, H., Kang, S. W., Chang, H., Ryu, J. H., Kwon, I. C., Cho, Y. W., and Kim, K. (2016). Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo. Bioconjug Chem 27, 927-936.
Yoshii, Y., Furukawa, T., Aoyama, H., Adachi, N., Zhang, M. R., Wakizaka, H., Fujibayashi, Y., and Saga, T. (2016). Regorafenib as a potential adjuvant chemotherapy agent in disseminated small colon cancer: Drug selection outcome of a novel screening system using nanoimprinting 3-dimensional culture with HCT116-RFP cells. Int J Oncol 48, 1477-1484.
Yu, T., Guo, Z., Fan, H., Song, J., Liu, Y., Gao, Z., and Wang, Q. (2016). Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device. Oncotarget.
Zanotelli, M. R., Ardalani, H., Zhang, J., Hou, Z., Nguyen, E. H., Swanson, S., Nguyen, B. K., Bolin, J., Elwell, A., Bischel, L. L., et al. (2016). Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels. Acta Biomater.
Zhang, X., Hu, M. G., Pan, K., Li, C. H., and Liu, R. (2016). 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis. Stem Cells Int 2016, 4626073.
Zheng, Y., Sun, Y., Yu, X., Shao, Y., Zhang, P., Dai, G., and Fu, J. (2016). Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis. Adv Healthc Mater.
Zhu, W., Ma, X., Gou, M., Mei, D., Zhang, K., and Chen, S. (2016). 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol 40, 103-112.
Reviews
Asthana, A., and Kisaalita, W. S. (2016). Molecular basis for cytokine biomarkers of complex 3D microtissue physiology in vitro. Drug Discov Today.
Barata, D., van Blitterswijk, C., and Habibovic, P. (2016). High-throughput screening approaches and combinatorial development of biomaterials using microfluidics. Acta Biomater 34, 1-20.
Chi, C. W., Ahmed, A. R., Dereli-Korkut, Z., and Wang, S. (2016). Microfluidic cell chips for high throughput drug screening. Bioanalysis, 0.
Emura, M., and Aufderheide, M. (2016). Challenge for 3D culture technology: Application in carcinogenesis studies with human airway epithelial cells. Exp Toxicol Pathol.
Follin, B., Juhl, M., Cohen, S., Perdersen, A. E., Kastrup, J., and Ekblond, A. (2016). Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture. Tissue Eng Part B Rev.
Gilmour, A. D., Woolley, A. J., Poole-Warren, L. A., Thomson, C. E., and Green, R. A. (2016). A critical review of cell culture strategies for modelling intracortical brain implant material reactions. Biomaterials 91, 23-43.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Katt, M. E., Placone, A. L., Wong, A. D., Xu, Z. S., and Searson, P. C. (2016). In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform. Front Bioeng Biotechnol 4, 12.
Keely, P., and Nain, A. (2015). Capturing relevant extracellular matrices for investigating cell migration. F1000Res 4.
Kim, J., and Tanner, K. (2016). Three-Dimensional Patterning of the ECM Microenvironment Using Magnetic Nanoparticle Self Assembly. Curr Protoc Cell Biol 70, 25.23.21-25.23.14.
Klotz, B. J., Gawlitta, D., Rosenberg, A. J., Malda, J., and Melchels, F. P. (2016). Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair. Trends Biotechnol.
Ko, K. R., and Frampton, J. P. (2016). Developments in 3D neural cell culture models: the future of neurotherapeutics testing? Expert Rev Neurother, 1-3.
Koutsopoulos, S. (2016). Self-assembling peptide nanofiber hydrogels in tissue engineering and regenerative medicine: Progress, design guidelines, and applications. J Biomed Mater Res A 104, 1002-1016.
Lei, Y., and Ferdous, Z. (2016). Design Considerations and Challenges for Mechanical Stretch Bioreactors in Tissue Engineering. Biotechnol Prog.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Pati, F., Gantelius, J., and Svahn, H. A. (2016). 3D Bioprinting of Tissue/Organ Models. Angew Chem Int Ed Engl.
Pellman, J., Zhang, J., and Sheikh, F. (2016). Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems. J Mol Cell Cardiol.
Ryan, S. L., Baird, A. M., Vaz, G., Urquhart, A. J., Senge, M., Richard, D. J., O'Byrne, K. J., and Davies, A. M. (2016). Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture. Assay Drug Dev Technol 14, 19-28.
Wang, Q. A., and Scherer, P. E. (2016). Human Beige Adipocytes: Epiphenomenon or Drivers of Metabolic Improvements? Trends Endocrinol Metab.
Zhu, W., Ma, X., Gou, M., Mei, D., Zhang, K., and Chen, S. (2016). 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol 40, 103-112.
Spheroids
Akasov, R., Zaytseva-Zotova, D., Burov, S., Leko, M., Dontenwill, M., Chiper, M., Vandamme, T., and Markvicheva, E. (2016). Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro. Int J Pharm.
Arevalos, C. A., Berg, J. M., Nguyen, J. M., Godfrey, E. L., Iriondo, C., and Grande-Allen, K. J. (2016). Valve Interstitial Cells Act in a Pericyte Manner Promoting Angiogensis and Invasion by Valve Endothelial Cells. Ann Biomed Eng.
Cardoso, T. C., Sakamoto, S. S., Stockmann, D., Souza, T. F., Ferreira, H. L., Gameiro, R., Vieira, F. V., Louzada, M. J., Andrade, A. L., and Flores, E. F. (2016). A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators. Vet Comp Oncol.
Cesarz, Z., Funnell, J. L., Guan, J., and Tamama, K. (2016). Soft Elasticity-Associated Signaling and Bone Morphogenic Protein 2 are Key Regulators of Mesenchymal Stem Cell Spheroidal Aggregates. Stem Cells Dev.
Ferraz, E. R., Rainho, C. R., Fernandes, A. S., and Felzenszwalb, I. (2016). Differential Toxicity of an Organic PM Extract to Human Lung Cells Cultured in Three Dimensions (3D) and Monolayers. J Toxicol Environ Health A, 1-11.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Jobe, N. P., Rosel, D., Dvorankova, B., Kodet, O., Lacina, L., Mateu, R., Smetana, K., and Brabek, J. (2016). Simultaneous blocking of IL-6 and IL-8 is sufficient to fully inhibit CAF-induced human melanoma cell invasiveness. Histochem Cell Biol.
Koeck, S., Zwierzina, M., Huber, J. M., Bitsche, M., Lorenz, E., Gamerith, G., Dudas, J., Kelm, J. M., Zwierzina, H., and Amann, A. (2016). Infiltration of lymphocyte subpopulations into cancer microtissues as a tool for the exploration of immunomodulatory agents and biomarkers. Immunobiology 221, 604-617.
Langan, L. M., Dodd, N. J., Owen, S. F., Purcell, W. M., Jackson, S. K., and Jha, A. N. (2016). Direct Measurements of Oxygen Gradients in Spheroid Culture System Using Electron Parametric Resonance Oximetry. PLoS One 11, e0149492.
Lapa, G. B., Tsunoda, T., Shirasawa, S., Baryshnikova, M. A., Evseev, G. G., Afanasyeva, D. A., and Chigorina, E. A. (2016). Synthesis of New Congeners of 1-methyl-3-aminoisoquinolines, Evaluation of Their Cytotoxic Activity, In Silico and In Vitro Study of Their Molecular Targets as PDE4B. Chem Biol Drug Des 87, 575-582.
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Lee, J. H., Lee, H. H., Kim, K. N., and Kim, K. M. (2016). Cytotoxicity and anti-inflammatory effects of zinc ions and eugenol during setting of ZOE in immortalized human oral keratinocytes grown as three-dimensional spheroids. Dent Mater.
Lin, B., Miao, Y., Wang, J., Fan, Z., Du, L., Su, Y., Liu, B., Hu, Z., and Xing, M. (2016). Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration. ACS Appl Mater Interfaces 8, 5906-5916.
Meenach, S. A., Tsoras, A. N., McGarry, R. C., Mansour, H. M., Hilt, J. Z., and Anderson, K. W. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. Int J Oncol 48, 1701-1709.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Patra, B., Peng, C. C., Liao, W. H., Lee, C. H., and Tung, Y. C. (2016). Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device. Sci Rep 6, 21061.
Relier, S., Yazdani, L., Ayad, O., Choquet, A., Bourgaux, J. F., Prudhomme, M., Pannequin, J., Macari, F., and David, A. (2016). Antibiotics inhibit sphere-forming ability in suspension culture. Cancer Cell Int 16, 6.
Ryan, S. L., Baird, A. M., Vaz, G., Urquhart, A. J., Senge, M., Richard, D. J., O'Byrne, K. J., and Davies, A. M. (2016). Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture. Assay Drug Dev Technol 14, 19-28.
Sarkar, J., and Kumar, A. (2016). Thermo-responsive polymer aided spheroid culture in cryogel based platform for high throughput drug screening. Analyst.
Sato, K., Choyke, P. L., and Hisataka, K. (2016). Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique. J Vis Exp.
Schmidt, M., Scholz, C. J., Polednik, C., and Roller, J. (2016). Spheroid-based 3-dimensional culture models: Gene expression and functionality in head and neck cancer. Oncol Rep 35, 2431-2440.
Shalaly, N. D., Ria, M., Johansson, U., Avall, K., Berggren, P. O., and Hedhammar, M. (2016). Silk matrices promote formation of insulin-secreting islet-like clusters. Biomaterials 90, 50-61.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Waisbourd-Zinman, O., Koh, H., Tsai, S., Lavrut, P. M., Dang, C., Zhao, X., Pack, M., Cave, J., Hawes, M., Koo, K. A., et al. (2016). The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis via decreased glutathione and SOX17. Hepatology.
Wang, J. Z., Zhu, Y. X., Ma, H. C., Chen, S. N., Chao, J. Y., Ruan, W. D., Wang, D., Du, F. G., and Meng, Y. Z. (2016). Developing multi-cellular tumor spheroid model (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. Mater Sci Eng C Mater Biol Appl 62, 215-225.
Xu, Y., Shi, T., Xu, A., and Zhang, L. (2016). 3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney. J Cell Mol Med.
Zhang, X., Hu, M. G., Pan, K., Li, C. H., and Liu, R. (2016). 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis. Stem Cells Int 2016, 4626073.
Organoid
Langan, L. M., Dodd, N. J., Owen, S. F., Purcell, W. M., Jackson, S. K., and Jha, A. N. (2016). Direct Measurements of Oxygen Gradients in Spheroid Culture System Using Electron Parametric Resonance Oximetry. PLoS One 11, e0149492.
Niu, C., Chauhan, U., Gargus, M., and Shaker, A. (2016). Generation and Characterization of an Immortalized Human Esophageal Myofibroblast Line. PLoS One 11, e0153185.
Otani, T., Marchetto, M. C., Gage, F. H., Simons, B. D., and Livesey, F. J. (2016). 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. Cell Stem Cell 18, 467-480.
Scaffold
Almela, T., Brook, I. M., and Moharamzadeh, K. (2016). Development of three-dimensional tissue engineered bone-oral mucosal composite models. J Mater Sci Mater Med 27, 65.
Alonso-Nocelo, M., Abuin, C., Lopez-Lopez, R., and de la Fuente, M. (2016). Development and characterization of a three-dimensional co-culture model of tumor T cell infiltration. Biofabrication 8, 025002.
Arnal-Pastor, M., Comin-Cebrian, S., Martinez-Ramos, C., Monleon Pradas, M., and Valles-Lluch, A. (2016). Hydrophilic surface modification of acrylate-based biomaterials. J Biomater Appl 30, 1429-1441.
Arslan, E., Guler, M. O., and Tekinay, A. B. (2016). Glycosaminoglycan-Mimetic Signals Direct the Osteo/Chondrogenic Differentiation of Mesenchymal Stem Cells in a Three-Dimensional Peptide Nanofiber Extracellular Matrix Mimetic Environment. Biomacromolecules 17, 1280-1291.
Asthana, A., and Kisaalita, W. S. (2016). Molecular basis for cytokine biomarkers of complex 3D microtissue physiology in vitro. Drug Discov Today.
Barata, D., van Blitterswijk, C., and Habibovic, P. (2016). High-throughput screening approaches and combinatorial development of biomaterials using microfluidics. Acta Biomater 34, 1-20.
Boyce, M. W., Kenney, R. M., Truong, A. S., and Lockett, M. R. (2016). Quantifying oxygen in paper-based cell cultures with luminescent thin film sensors. Anal Bioanal Chem 408, 2985-2992.
Cardoso, T. C., Sakamoto, S. S., Stockmann, D., Souza, T. F., Ferreira, H. L., Gameiro, R., Vieira, F. V., Louzada, M. J., Andrade, A. L., and Flores, E. F. (2016). A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators. Vet Comp Oncol.
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Matrix
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Arslan, E., Guler, M. O., and Tekinay, A. B. (2016). Glycosaminoglycan-Mimetic Signals Direct the Osteo/Chondrogenic Differentiation of Mesenchymal Stem Cells in a Three-Dimensional Peptide Nanofiber Extracellular Matrix Mimetic Environment. Biomacromolecules 17, 1280-1291.
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Casey, A., Gargotti, M., Bonnier, F., and Byrne, H. J. (2016). Chemotherapeutic efficiency of drugs in vitro: Comparison of doxorubicin exposure in 3D and 2D culture matrices. Toxicol In Vitro 33, 99-104.
Chamorro, C. I., Zeiai, S., Reinfeldt Engberg, G., and Fossum, M. (2016). Minced Tissue in Compressed Collagen: A Cell-containing Biotransplant for Single-staged Reconstructive Repair. J Vis Exp.
Chen, W., Ma, J., Zhu, L., Morsi, Y., Ei-Hamshary, H., Al-Deyab, S. S., and Mo, X. (2016). Superelastic, superabsorbent and 3D nanofiber-assembled scaffold for tissue engineering. Colloids Surf B Biointerfaces 142, 165-172.
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Dehdilani, N., Shamsasenjan, K., Movassaghpour, A., Akbarzadehlaleh, P., Amoughli Tabrizi, B., Parsa, H., and Sabagi, F. (2016). Improved Survival and Hematopoietic Differentiation of Murine Embryonic Stem Cells on Electrospun Polycaprolactone Nanofiber. Cell J 17, 629-638.
Duan, B., Yin, Z., Hockaday Kang, L., Magin, R. L., and Butcher, J. T. (2016). Active tissue stiffness modulation controls valve interstitial cell phenotype and osteogenic potential in 3D culture. Acta Biomater.
Gonzalez, S., Mei, H., Nakatsu, M. N., Baclagon, E. R., and Deng, S. X. (2016). A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16, 358-364.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Harris, S., Wegner, S., Hong, S. W., and Faustman, E. M. (2016). Phthalate metabolism and kinetics in an in vitro model of testis development. Toxicol In Vitro 32, 123-131.
He, P., Fu, J., and Wang, D. A. (2016). Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform. Acta Biomater.
Hof, A., Raschke, S., Baier, K., Nehrenheim, L., Selig, J. I., Schomaker, M., Lichtenberg, A., Meyer, H., and Akhyari, P. (2016). Challenges in developing a reseeded, tissue-engineered aortic valve prosthesis. Eur J Cardiothorac Surg.
Huang, Z., Zhen, Y., Yin, W., Ma, Z., and Zhang, L. (2016). Shh promotes sweat gland cell maturation in three-dimensional culture. Cell Tissue Bank.
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Klotz, B. J., Gawlitta, D., Rosenberg, A. J., Malda, J., and Melchels, F. P. (2016). Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair. Trends Biotechnol.
Labour, M. N., Vigier, S., Lerner, D., Marcilhac, A., and Belamie, E. (2016). 3D compartmented model to study the neurite-related toxicity of Abeta aggregates included in collagen gels of adaptable porosity. Acta Biomater.
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Li, Y., Huang, G., Li, M., Wang, L., Elson, E. L., Jian Lu, T., Genin, G. M., and Xu, F. (2016). An approach to quantifying 3D responses of cells to extreme strain. Sci Rep 6, 19550.
Lu, L., and Unsworth, L. D. (2016). pH-Triggered Release of Hydrophobic Molecules from Self-Assembling Hybrid Nanoscaffolds. Biomacromolecules.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Ning, L., Xu, Y., Chen, X., and Schreyer, D. J. (2016). Influence of mechanical properties of alginate-based substrates on the performance of Schwann cells in culture. J Biomater Sci Polym Ed, 1-18.
Parvizi, M., Bolhuis-Versteeg, L. A., Poot, A. A., and Harmsen, M. C. (2016). Efficient generation of smooth muscle cells from adipose-derived stromal cells by 3D mechanical stimulation can substitute the use of growth factors in vascular tissue engineering. Biotechnol J.
Pascu, E. I., Cahill, P. A., Stokes, J., and McGuinness, G. B. (2016). Towards functional 3D-stacked electrospun composite scaffolds of PHBV, silk fibroin and nanohydroxyapatite: Mechanical properties and surface osteogenic differentiation. J Biomater Appl 30, 1334-1349.
Pellman, J., Zhang, J., and Sheikh, F. (2016). Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems. J Mol Cell Cardiol.
Penolazzi, L., Lolli, A., Sardelli, L., Angelozzi, M., Lambertini, E., Trombelli, L., Ciarpella, F., RenataVecchiatini, and Piva, R. (2016). Establishment of a 3D-dynamic osteoblasts-osteoclasts co-culture model to simulate the jawbone microenvironment in vitro. Life Sci.
Petropolis, D. B., Faust, D. M., Tolle, M., Riviere, L., Valentin, T., Neuveut, C., Hernandez-Cuevas, N., Dufour, A., Olivo-Marin, J. C., and Guillen, N. (2016). Human Liver Infection in a Dish: Easy-To-Build 3D Liver Models for Studying Microbial Infection. PLoS One 11, e0148667.
Pouliot, R. A., Link, P. A., Mikhaiel, N. S., Schneck, M. B., Valentine, M. S., Kamga Gninzeko, F. J., Herbert, J. A., Sakagami, M., and Heise, R. L. (2016). Development and characterization of a naturally derived lung extracellular matrix hydrogel. J Biomed Mater Res A.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Raghothaman, D., Leong, M. F., Lim, T. C., Wan, A. C., Ser, Z., Lee, E. H., and Yang, Z. (2016). Cell type dependent morphological adaptation in polyelectrolyte hydrogels governs chondrogenic fate. Biomed Mater 11, 025013.
Recha-Sancho, L., and Semino, C. E. (2016). Heparin based self-assembling peptide scaffold reestablish chondrogenic phenotype of expanded de-differentiated human chondrocytes. J Biomed Mater Res A.
Shalaly, N. D., Ria, M., Johansson, U., Avall, K., Berggren, P. O., and Hedhammar, M. (2016). Silk matrices promote formation of insulin-secreting islet-like clusters. Biomaterials 90, 50-61.
Simao, D., Arez, F., Terasso, A. P., Pinto, C., Sousa, M. F., Brito, C., and Alves, P. M. (2016). Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells. Methods Mol Biol.
Sokol, E. S., Miller, D. H., Breggia, A., Spencer, K. C., Arendt, L. M., and Gupta, P. B. (2016). Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Breast Cancer Res 18, 19.
Speroni, L., Sweeney, M. F., Sonnenschein, C., and Soto, A. M. (2016). A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium. J Vis Exp.
Suntornnond, R., An, J., Tijore, A., Leong, K. F., Chua, C. K., and Tan, L. P. (2016). A Solvent-Free Surface Suspension Melt Technique for Making Biodegradable PCL Membrane Scaffolds for Tissue Engineering Applications. Molecules 21.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Tonello, S., Moore, M. C., Sharma, B., Dobson, J., and McFetridge, P. S. (2016). Controlled release of a heterogeneous human placental matrix from PLGA microparticles to modulate angiogenesis. Drug Deliv Transl Res 6, 174-183.
Weber, L., Langer, M., Tavella, S., Ruggiu, A., and Peyrin, F. (2016). Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells. Phys Med Biol 61, N215-n231.
Xu, Y., Shi, T., Xu, A., and Zhang, L. (2016). 3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney. J Cell Mol Med.
Zanotelli, M. R., Ardalani, H., Zhang, J., Hou, Z., Nguyen, E. H., Swanson, S., Nguyen, B. K., Bolin, J., Elwell, A., Bischel, L. L., et al. (2016). Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels. Acta Biomater.
Microfluidics
Alessandri, K., Feyeux, M., Gurchenkov, B., Delgado, C., Trushko, A., Krause, K. H., Vignjevic, D., Nassoy, P., and Roux, A. (2016). A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC). Lab Chip.
Barata, D., van Blitterswijk, C., and Habibovic, P. (2016). High-throughput screening approaches and combinatorial development of biomaterials using microfluidics. Acta Biomater 34, 1-20.
Chi, C. W., Ahmed, A. R., Dereli-Korkut, Z., and Wang, S. (2016). Microfluidic cell chips for high throughput drug screening. Bioanalysis, 0.
Choi, C. H., Wang, H., Lee, H., Kim, J. H., Zhang, L., Mao, A., Mooney, D. J., and Weitz, D. A. (2016). One-step generation of cell-laden microgels using double emulsion drops with a sacrificial ultra-thin oil shell. Lab Chip.
Christoffersson, J., Bergstrom, G., Schwanke, K., Kempf, H., Zweigerdt, R., and Mandenius, C. F. (2016). A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies. Methods Mol Biol.
Gilmour, A. D., Woolley, A. J., Poole-Warren, L. A., Thomson, C. E., and Green, R. A. (2016). A critical review of cell culture strategies for modelling intracortical brain implant material reactions. Biomaterials 91, 23-43.
Patra, B., Peng, C. C., Liao, W. H., Lee, C. H., and Tung, Y. C. (2016). Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device. Sci Rep 6, 21061.
Ramadan, Q., and Ting, F. C. (2016). In vitro micro-physiological immune-competent model of the human skin. Lab Chip.
Yu, T., Guo, Z., Fan, H., Song, J., Liu, Y., Gao, Z., and Wang, Q. (2016). Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device. Oncotarget.
Zanotelli, M. R., Ardalani, H., Zhang, J., Hou, Z., Nguyen, E. H., Swanson, S., Nguyen, B. K., Bolin, J., Elwell, A., Bischel, L. L., et al. (2016). Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels. Acta Biomater.
Microfabrication
Dai, G., Wan, W., Zhao, Y., Wang, Z., Li, W., Shi, P., and Shen, Y. (2016). Controllable 3D alginate hydrogel patterning via visible-light induced electrodeposition. Biofabrication 8, 025004.
Katt, M. E., Placone, A. L., Wong, A. D., Xu, Z. S., and Searson, P. C. (2016). In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform. Front Bioeng Biotechnol 4, 12.
Patel, N. M., Yazdi, I. K., Tasciotti, E., and Birla, R. K. (2016). Optimizing cell seeding and retention in a three-dimensional bioengineered cardiac ventricle: The two-stage cellularization model. Biotechnol Bioeng.
Adipocyte
Wang, Q. A., and Scherer, P. E. (2016). Human Beige Adipocytes: Epiphenomenon or Drivers of Metabolic Improvements? Trends Endocrinol Metab.
Bone
Albritton, J. L., Roybal, J. D., Paulsen, S. J., Calafat, N., Flores-Zaher, J. A., Farach-Carson, M. C., Gibbons, D. L., and Miller, J. S. (2016). Ultrahigh-throughput Generation and Characterization of Cellular Aggregates in Laser-ablated Microwells of Poly(dimethylsiloxane). RSC Adv 6, 8980-8991.
Almela, T., Brook, I. M., and Moharamzadeh, K. (2016). Development of three-dimensional tissue engineered bone-oral mucosal composite models. J Mater Sci Mater Med 27, 65.
Calabrese, R., Raia, N., Huang, W., Ghezzi, C. E., Simon, M., Staii, C., Weiss, A. S., and Kaplan, D. L. (2016). Silk-ionomer and silk-tropoelastin hydrogels as charged three-dimensional culture platforms for the regulation of hMSC response. J Tissue Eng Regen Med.
Cesarz, Z., Funnell, J. L., Guan, J., and Tamama, K. (2016). Soft Elasticity-Associated Signaling and Bone Morphogenic Protein 2 are Key Regulators of Mesenchymal Stem Cell Spheroidal Aggregates. Stem Cells Dev.
Duarte Campos, D. F., Blaeser, A., Buellesbach, K., Sen, K. S., Xun, W., Tillmann, W., and Fischer, H. (2016). Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering. Adv Healthc Mater.
Gonzalez, S., Mei, H., Nakatsu, M. N., Baclagon, E. R., and Deng, S. X. (2016). A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16, 358-364.
Han, S., Wang, B., Li, X., Xiao, Z., Han, J., Zhao, Y., Fang, Y., Yin, Y., Chen, B., and Dai, J. (2016). Bone marrow-derived mesenchymal stem cells in three-dimensional culture promote neuronal regeneration by neurotrophic protection and immunomodulation. J Biomed Mater Res A.
Huang, Z., Zhen, Y., Yin, W., Ma, Z., and Zhang, L. (2016). Shh promotes sweat gland cell maturation in three-dimensional culture. Cell Tissue Bank.
Katti, K. S., Molla, S., Karandish, F., Haldar, M. K., Mallik, S., and Katti, D. R. (2016). Sequential culture on biomimetic nanoclay scaffolds forms three dimensional tumoroids. J Biomed Mater Res A.
Klotz, B. J., Gawlitta, D., Rosenberg, A. J., Malda, J., and Melchels, F. P. (2016). Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair. Trends Biotechnol.
Nowotny, J., Aibibu, D., Farack, J., Nimtschke, U., Hild, M., Gelinsky, M., Kasten, P., and Cherif, C. (2016). Novel fiber-based pure chitosan scaffold for tendon augmentation: biomechanical and cell biological evaluation. J Biomater Sci Polym Ed, 1-20.
Pascu, E. I., Cahill, P. A., Stokes, J., and McGuinness, G. B. (2016). Towards functional 3D-stacked electrospun composite scaffolds of PHBV, silk fibroin and nanohydroxyapatite: Mechanical properties and surface osteogenic differentiation. J Biomater Appl 30, 1334-1349.
Penolazzi, L., Lolli, A., Sardelli, L., Angelozzi, M., Lambertini, E., Trombelli, L., Ciarpella, F., RenataVecchiatini, and Piva, R. (2016). Establishment of a 3D-dynamic osteoblasts-osteoclasts co-culture model to simulate the jawbone microenvironment in vitro. Life Sci.
Tutak, W., Jyotsnendu, G., Bajcsy, P., and Simon, C. G., Jr. (2016). Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. J Biomed Mater Res B Appl Biomater.
Wang, H., Wu, G., Zhang, J., Zhou, K., Yin, B., Su, X., Qiu, G., Yang, G., Zhang, X., Zhou, G., and Wu, Z. (2016). Osteogenic effect of controlled released rhBMP-2 in 3D printed porous hydroxyapatite scaffold. Colloids Surf B Biointerfaces 141, 491-498.
Weber, L., Langer, M., Tavella, S., Ruggiu, A., and Peyrin, F. (2016). Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells. Phys Med Biol 61, N215-n231.
Yassin, M. A., Leknes, K. N., Sun, Y., Lie, S. A., Finne-Wistrand, A., and Mustafa, K. (2016). Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation. J Biomed Mater Res A.
Ye, J., Wang, J., Zhu, Y., Wei, Q., Wang, X., Yang, J., Tang, S., Liu, H., Fan, J., Zhang, F., et al. (2016). A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells. Biomed Mater 11, 025021.
Yoon, H. I., Yhee, J. Y., Na, J. H., Lee, S., Lee, H., Kang, S. W., Chang, H., Ryu, J. H., Kwon, I. C., Cho, Y. W., and Kim, K. (2016). Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo. Bioconjug Chem 27, 927-936.
Zheng, Y., Sun, Y., Yu, X., Shao, Y., Zhang, P., Dai, G., and Fu, J. (2016). Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis. Adv Healthc Mater.
Bone Marrow
Albritton, J. L., Roybal, J. D., Paulsen, S. J., Calafat, N., Flores-Zaher, J. A., Farach-Carson, M. C., Gibbons, D. L., and Miller, J. S. (2016). Ultrahigh-throughput Generation and Characterization of Cellular Aggregates in Laser-ablated Microwells of Poly(dimethylsiloxane). RSC Adv 6, 8980-8991.
Almela, T., Brook, I. M., and Moharamzadeh, K. (2016). Development of three-dimensional tissue engineered bone-oral mucosal composite models. J Mater Sci Mater Med 27, 65.
Calabrese, R., Raia, N., Huang, W., Ghezzi, C. E., Simon, M., Staii, C., Weiss, A. S., and Kaplan, D. L. (2016). Silk-ionomer and silk-tropoelastin hydrogels as charged three-dimensional culture platforms for the regulation of hMSC response. J Tissue Eng Regen Med.
Cesarz, Z., Funnell, J. L., Guan, J., and Tamama, K. (2016). Soft Elasticity-Associated Signaling and Bone Morphogenic Protein 2 are Key Regulators of Mesenchymal Stem Cell Spheroidal Aggregates. Stem Cells Dev.
Duarte Campos, D. F., Blaeser, A., Buellesbach, K., Sen, K. S., Xun, W., Tillmann, W., and Fischer, H. (2016). Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering. Adv Healthc Mater.
Gonzalez, S., Mei, H., Nakatsu, M. N., Baclagon, E. R., and Deng, S. X. (2016). A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16, 358-364.
Han, S., Wang, B., Li, X., Xiao, Z., Han, J., Zhao, Y., Fang, Y., Yin, Y., Chen, B., and Dai, J. (2016). Bone marrow-derived mesenchymal stem cells in three-dimensional culture promote neuronal regeneration by neurotrophic protection and immunomodulation. J Biomed Mater Res A.
Huang, Z., Zhen, Y., Yin, W., Ma, Z., and Zhang, L. (2016). Shh promotes sweat gland cell maturation in three-dimensional culture. Cell Tissue Bank.
Katti, K. S., Molla, S., Karandish, F., Haldar, M. K., Mallik, S., and Katti, D. R. (2016). Sequential culture on biomimetic nanoclay scaffolds forms three dimensional tumoroids. J Biomed Mater Res A.
Klotz, B. J., Gawlitta, D., Rosenberg, A. J., Malda, J., and Melchels, F. P. (2016). Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair. Trends Biotechnol.
Nowotny, J., Aibibu, D., Farack, J., Nimtschke, U., Hild, M., Gelinsky, M., Kasten, P., and Cherif, C. (2016). Novel fiber-based pure chitosan scaffold for tendon augmentation: biomechanical and cell biological evaluation. J Biomater Sci Polym Ed, 1-20.
Pascu, E. I., Cahill, P. A., Stokes, J., and McGuinness, G. B. (2016). Towards functional 3D-stacked electrospun composite scaffolds of PHBV, silk fibroin and nanohydroxyapatite: Mechanical properties and surface osteogenic differentiation. J Biomater Appl 30, 1334-1349.
Penolazzi, L., Lolli, A., Sardelli, L., Angelozzi, M., Lambertini, E., Trombelli, L., Ciarpella, F., RenataVecchiatini, and Piva, R. (2016). Establishment of a 3D-dynamic osteoblasts-osteoclasts co-culture model to simulate the jawbone microenvironment in vitro. Life Sci.
Tutak, W., Jyotsnendu, G., Bajcsy, P., and Simon, C. G., Jr. (2016). Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. J Biomed Mater Res B Appl Biomater.
Wang, H., Wu, G., Zhang, J., Zhou, K., Yin, B., Su, X., Qiu, G., Yang, G., Zhang, X., Zhou, G., and Wu, Z. (2016). Osteogenic effect of controlled released rhBMP-2 in 3D printed porous hydroxyapatite scaffold. Colloids Surf B Biointerfaces 141, 491-498.
Weber, L., Langer, M., Tavella, S., Ruggiu, A., and Peyrin, F. (2016). Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells. Phys Med Biol 61, N215-n231.
Yassin, M. A., Leknes, K. N., Sun, Y., Lie, S. A., Finne-Wistrand, A., and Mustafa, K. (2016). Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation. J Biomed Mater Res A.
Ye, J., Wang, J., Zhu, Y., Wei, Q., Wang, X., Yang, J., Tang, S., Liu, H., Fan, J., Zhang, F., et al. (2016). A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells. Biomed Mater 11, 025021.
Yoon, H. I., Yhee, J. Y., Na, J. H., Lee, S., Lee, H., Kang, S. W., Chang, H., Ryu, J. H., Kwon, I. C., Cho, Y. W., and Kim, K. (2016). Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo. Bioconjug Chem 27, 927-936.
Zheng, Y., Sun, Y., Yu, X., Shao, Y., Zhang, P., Dai, G., and Fu, J. (2016). Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis. Adv Healthc Mater.
Breast
Boyce, M. W., Kenney, R. M., Truong, A. S., and Lockett, M. R. (2016). Quantifying oxygen in paper-based cell cultures with luminescent thin film sensors. Anal Bioanal Chem 408, 2985-2992.
Itou, J., Tanaka, S., Li, W., Matsumoto, Y., Sato, F., and Toi, M. (2015). Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells. Data Brief 5, 429-433.
Lapa, G. B., Tsunoda, T., Shirasawa, S., Baryshnikova, M. A., Evseev, G. G., Afanasyeva, D. A., and Chigorina, E. A. (2016). Synthesis of New Congeners of 1-methyl-3-aminoisoquinolines, Evaluation of Their Cytotoxic Activity, In Silico and In Vitro Study of Their Molecular Targets as PDE4B. Chem Biol Drug Des 87, 575-582.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Oldenburg, A. L., Yu, X., Gilliss, T., Alabi, O., Taylor, R. M., 2nd, and Troester, M. A. (2015). Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy. Optica 2, 877-885.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Sarkar, J., and Kumar, A. (2016). Thermo-responsive polymer aided spheroid culture in cryogel based platform for high throughput drug screening. Analyst.
Sokol, E. S., Miller, D. H., Breggia, A., Spencer, K. C., Arendt, L. M., and Gupta, P. B. (2016). Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Breast Cancer Res 18, 19.
Speroni, L., Sweeney, M. F., Sonnenschein, C., and Soto, A. M. (2016). A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium. J Vis Exp.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Vantangoli, M. M., Wilson, S., Madnick, S. J., Huse, S. M., and Boekelheide, K. (2016). Morphologic effects of estrogen stimulation on 3D MCF-7 microtissues. Toxicol Lett 248, 1-8.
Weigand, A., Boos, A. M., Tasbihi, K., Beier, J. P., Dalton, P. D., Schrauder, M., Horch, R. E., Beckmann, M. W., Strissel, P. L., and Strick, R. (2016). Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells. Breast Cancer Res 18, 32.
Xu, H., Liu, W., Zhang, X. Z., Hou, L., Lu, Y. J., Chen, P. P., Zhang, C., Feng, D., Kong, L., and Wang, X. L. (2016). [Development of three-dimensional breast cancer cell culture drug resistance model]. Sheng Li Xue Bao 68, 179-184.
Colon
Yoshii, Y., Furukawa, T., Aoyama, H., Adachi, N., Zhang, M. R., Wakizaka, H., Fujibayashi, Y., and Saga, T. (2016). Regorafenib as a potential adjuvant chemotherapy agent in disseminated small colon cancer: Drug selection outcome of a novel screening system using nanoimprinting 3-dimensional culture with HCT116-RFP cells. Int J Oncol 48, 1477-1484.
Heart
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Duan, B., Yin, Z., Hockaday Kang, L., Magin, R. L., and Butcher, J. T. (2016). Active tissue stiffness modulation controls valve interstitial cell phenotype and osteogenic potential in 3D culture. Acta Biomater.
Fischer, K. M., Morgan, K. Y., Hearon, K., Sklaviadis, D., Tochka, Z. L., Fenton, O. S., Anderson, D. G., Langer, R., and Freed, L. E. (2016). Poly(Limonene Thioether) Scaffold for Tissue Engineering. Adv Healthc Mater.
Follin, B., Juhl, M., Cohen, S., Perdersen, A. E., Kastrup, J., and Ekblond, A. (2016). Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture. Tissue Eng Part B Rev.
Hof, A., Raschke, S., Baier, K., Nehrenheim, L., Selig, J. I., Schomaker, M., Lichtenberg, A., Meyer, H., and Akhyari, P. (2016). Challenges in developing a reseeded, tissue-engineered aortic valve prosthesis. Eur J Cardiothorac Surg.
Kang, L. H., Armstrong, P. A., Lee, L. J., Duan, B., Kang, K. H., and Butcher, J. T. (2016). Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels. Ann Biomed Eng.
Lei, Y., and Ferdous, Z. (2016). Design Considerations and Challenges for Mechanical Stretch Bioreactors in Tissue Engineering. Biotechnol Prog.
Patel, N. M., Yazdi, I. K., Tasciotti, E., and Birla, R. K. (2016). Optimizing cell seeding and retention in a three-dimensional bioengineered cardiac ventricle: The two-stage cellularization model. Biotechnol Bioeng.
Pellman, J., Zhang, J., and Sheikh, F. (2016). Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems. J Mol Cell Cardiol.
Liver
Langan, L. M., Dodd, N. J., Owen, S. F., Purcell, W. M., Jackson, S. K., and Jha, A. N. (2016). Direct Measurements of Oxygen Gradients in Spheroid Culture System Using Electron Parametric Resonance Oximetry. PLoS One 11, e0149492.
Liu, Z., Takeuchi, M., Nakajima, M., Hasegawa, Y., Huang, Q., and Fukuda, T. (2016). Shape-controlled high cell-density microcapsules by electrodeposition. Acta Biomater.
Luckert, C., Schulz, C., Lehmann, N., Thomas, M., Hofmann, U., Hammad, S., Hengstler, J. G., Braeuning, A., Lampen, A., and Hessel, S. (2016). Comparative analysis of 3D culture methods on human HepG2 cells. Arch Toxicol.
Petropolis, D. B., Faust, D. M., Tolle, M., Riviere, L., Valentin, T., Neuveut, C., Hernandez-Cuevas, N., Dufour, A., Olivo-Marin, J. C., and Guillen, N. (2016). Human Liver Infection in a Dish: Easy-To-Build 3D Liver Models for Studying Microbial Infection. PLoS One 11, e0148667.
Waisbourd-Zinman, O., Koh, H., Tsai, S., Lavrut, P. M., Dang, C., Zhao, X., Pack, M., Cave, J., Hawes, M., Koo, K. A., et al. (2016). The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis via decreased glutathione and SOX17. Hepatology.
Wang, J., Chen, F., Liu, L., Qi, C., Wang, B., Yan, X., Huang, C., Hou, W., Zhang, M. Q., Chen, Y., and Du, Y. (2016). Engineering EMT using 3D micro-scaffold to promote hepatic functions for drug hepatotoxicity evaluation. Biomaterials 91, 11-22.
Zhang, X., Hu, M. G., Pan, K., Li, C. H., and Liu, R. (2016). 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis. Stem Cells Int 2016, 4626073.
Lung
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Emura, M., and Aufderheide, M. (2016). Challenge for 3D culture technology: Application in carcinogenesis studies with human airway epithelial cells. Exp Toxicol Pathol.
Ferraz, E. R., Rainho, C. R., Fernandes, A. S., and Felzenszwalb, I. (2016). Differential Toxicity of an Organic PM Extract to Human Lung Cells Cultured in Three Dimensions (3D) and Monolayers. J Toxicol Environ Health A, 1-11.
Kato, T., Oka, K., Nakamura, T., and Ito, A. (2016). Decreased expression of Met during differentiation in rat lung. Eur J Histochem 60, 2575.
Koeck, S., Zwierzina, M., Huber, J. M., Bitsche, M., Lorenz, E., Gamerith, G., Dudas, J., Kelm, J. M., Zwierzina, H., and Amann, A. (2016). Infiltration of lymphocyte subpopulations into cancer microtissues as a tool for the exploration of immunomodulatory agents and biomarkers. Immunobiology 221, 604-617.
Meenach, S. A., Tsoras, A. N., McGarry, R. C., Mansour, H. M., Hilt, J. Z., and Anderson, K. W. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. Int J Oncol 48, 1701-1709.
O'Leary, C., Cavanagh, B., Unger, R. E., Kirkpatrick, C. J., O'Dea, S., O'Brien, F. J., and Cryan, S. A. (2016). The development of a tissue-engineered tracheobronchial epithelial model using a bilayered collagen-hyaluronate scaffold. Biomaterials 85, 111-127.
Pouliot, R. A., Link, P. A., Mikhaiel, N. S., Schneck, M. B., Valentine, M. S., Kamga Gninzeko, F. J., Herbert, J. A., Sakagami, M., and Heise, R. L. (2016). Development and characterization of a naturally derived lung extracellular matrix hydrogel. J Biomed Mater Res A.
Sarkar, J., and Kumar, A. (2016). Thermo-responsive polymer aided spheroid culture in cryogel based platform for high throughput drug screening. Analyst.
Yu, T., Guo, Z., Fan, H., Song, J., Liu, Y., Gao, Z., and Wang, Q. (2016). Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device. Oncotarget.
Muscle
Bono, N., Pezzoli, D., Levesque, L., Loy, C., Candiani, G., Fiore, G. B., and Mantovani, D. (2016). Unraveling the role of mechanical stimulation on smooth muscle cells: A comparative study between 2D and 3D models. Biotechnol Bioeng.
Kang, L. H., Armstrong, P. A., Lee, L. J., Duan, B., Kang, K. H., and Butcher, J. T. (2016). Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels. Ann Biomed Eng.
Lin, B., Miao, Y., Wang, J., Fan, Z., Du, L., Su, Y., Liu, B., Hu, Z., and Xing, M. (2016). Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration. ACS Appl Mater Interfaces 8, 5906-5916.
Parvizi, M., Bolhuis-Versteeg, L. A., Poot, A. A., and Harmsen, M. C. (2016). Efficient generation of smooth muscle cells from adipose-derived stromal cells by 3D mechanical stimulation can substitute the use of growth factors in vascular tissue engineering. Biotechnol J.
Patel, N. M., Yazdi, I. K., Tasciotti, E., and Birla, R. K. (2016). Optimizing cell seeding and retention in a three-dimensional bioengineered cardiac ventricle: The two-stage cellularization model. Biotechnol Bioeng.
Pinnock, C. B., Meier, E. M., Joshi, N. N., Wu, B., and Lam, M. T. (2016). Customizable engineered blood vessels using 3D printed inserts. Methods 99, 20-27.
Waisbourd-Zinman, O., Koh, H., Tsai, S., Lavrut, P. M., Dang, C., Zhao, X., Pack, M., Cave, J., Hawes, M., Koo, K. A., et al. (2016). The toxin biliatresone causes mouse extrahepatic cholangiocyte damage and fibrosis via decreased glutathione and SOX17. Hepatology
Nerve
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Prostate
Albritton, J. L., Roybal, J. D., Paulsen, S. J., Calafat, N., Flores-Zaher, J. A., Farach-Carson, M. C., Gibbons, D. L., and Miller, J. S. (2016). Ultrahigh-throughput Generation and Characterization of Cellular Aggregates in Laser-ablated Microwells of Poly(dimethylsiloxane). RSC Adv 6, 8980-8991.
Katti, K. S., Molla, S., Karandish, F., Haldar, M. K., Mallik, S., and Katti, D. R. (2016). Sequential culture on biomimetic nanoclay scaffolds forms three dimensional tumoroids. J Biomed Mater Res A.
Loessner, D., Meinert, C., Kaemmerer, E., Martine, L. C., Yue, K., Levett, P. A., Klein, T. J., Melchels, F. P., Khademhosseini, A., and Hutmacher, D. W. (2016). Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. Nat Protoc 11, 727-746.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Endothelial cells
Arevalos, C. A., Berg, J. M., Nguyen, J. M., Godfrey, E. L., Iriondo, C., and Grande-Allen, K. J. (2016). Valve Interstitial Cells Act in a Pericyte Manner Promoting Angiogensis and Invasion by Valve Endothelial Cells. Ann Biomed Eng.
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Li, C. W., Pan, W. T., Ju, J. C., and Wang, G. J. (2016). An endothelial cultured condition medium embedded porous PLGA scaffold for the enhancement of mouse embryonic stem cell differentiation. Biomed Mater 11, 025015.
McConkey, C. A., Delorme-Axford, E., Nickerson, C. A., Kim, K. S., Sadovsky, Y., Boyle, J. P., and Coyne, C. B. (2016). A three-dimensional culture system recapitulates placental syncytiotrophoblast development and microbial resistance. Sci Adv 2, e1501462.
Petropolis, D. B., Faust, D. M., Tolle, M., Riviere, L., Valentin, T., Neuveut, C., Hernandez-Cuevas, N., Dufour, A., Olivo-Marin, J. C., and Guillen, N. (2016). Human Liver Infection in a Dish: Easy-To-Build 3D Liver Models for Studying Microbial Infection. PLoS One 11, e0148667.
Robinson, S. T., Douglas, A. M., Chadid, T., Kuo, K., Rajabalan, A., Li, H., Copland, I. B., Barker, T. H., Galipeau, J., and Brewster, L. P. (2016). A Novel Platelet Lysate Hydrogel for Endothelial Cell and Mesenchymal Stem Cell-Directed Neovascularization. Acta Biomater.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Tonello, S., Moore, M. C., Sharma, B., Dobson, J., and McFetridge, P. S. (2016). Controlled release of a heterogeneous human placental matrix from PLGA microparticles to modulate angiogenesis. Drug Deliv Transl Res 6, 174-183.
Weigand, A., Boos, A. M., Tasbihi, K., Beier, J. P., Dalton, P. D., Schrauder, M., Horch, R. E., Beckmann, M. W., Strissel, P. L., and Strick, R. (2016). Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells. Breast Cancer Res 18, 32.
Xie, Q., Xie, J., Zhong, J., Cun, X., Lin, S., Lin, Y., and Cai, X. (2016). Hypoxia enhances angiogenesis in an adipose-derived stromal cell/endothelial cell co-culture 3D gel model. Cell Prolif.
Ye, J., Wang, J., Zhu, Y., Wei, Q., Wang, X., Yang, J., Tang, S., Liu, H., Fan, J., Zhang, F., et al. (2016). A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells. Biomed Mater 11, 025021.
Zanotelli, M. R., Ardalani, H., Zhang, J., Hou, Z., Nguyen, E. H., Swanson, S., Nguyen, B. K., Bolin, J., Elwell, A., Bischel, L. L., et al. (2016). Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels. Acta Biomater.
Zheng, Y., Sun, Y., Yu, X., Shao, Y., Zhang, P., Dai, G., and Fu, J. (2016). Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis. Adv Healthc Mater.
Fibroblast
Pellman, J., Zhang, J., and Sheikh, F. (2016). Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: Mechanisms and model systems. J Mol Cell Cardiol.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Xu, Y., Shi, T., Xu, A., and Zhang, L. (2016). 3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney. J Cell Mol Med.
Stem Cells
Albritton, J. L., Roybal, J. D., Paulsen, S. J., Calafat, N., Flores-Zaher, J. A., Farach-Carson, M. C., Gibbons, D. L., and Miller, J. S. (2016). Ultrahigh-throughput Generation and Characterization of Cellular Aggregates in Laser-ablated Microwells of Poly(dimethylsiloxane). RSC Adv 6, 8980-8991.
Alessandri, K., Feyeux, M., Gurchenkov, B., Delgado, C., Trushko, A., Krause, K. H., Vignjevic, D., Nassoy, P., and Roux, A. (2016). A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC). Lab Chip.
Arslan, E., Guler, M. O., and Tekinay, A. B. (2016). Glycosaminoglycan-Mimetic Signals Direct the Osteo/Chondrogenic Differentiation of Mesenchymal Stem Cells in a Three-Dimensional Peptide Nanofiber Extracellular Matrix Mimetic Environment. Biomacromolecules 17, 1280-1291.
Bellotti, C., Duchi, S., Bevilacqua, A., Lucarelli, E., and Piccinini, F. (2016). Long term morphological characterization of mesenchymal stromal cells 3D spheroids built with a rapid method based on entry-level equipment. Cytotechnology.
Calabrese, R., Raia, N., Huang, W., Ghezzi, C. E., Simon, M., Staii, C., Weiss, A. S., and Kaplan, D. L. (2016). Silk-ionomer and silk-tropoelastin hydrogels as charged three-dimensional culture platforms for the regulation of hMSC response. J Tissue Eng Regen Med.
Cesarz, Z., Funnell, J. L., Guan, J., and Tamama, K. (2016). Soft Elasticity-Associated Signaling and Bone Morphogenic Protein 2 are Key Regulators of Mesenchymal Stem Cell Spheroidal Aggregates. Stem Cells Dev.
Christoffersson, J., Bergstrom, G., Schwanke, K., Kempf, H., Zweigerdt, R., and Mandenius, C. F. (2016). A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies. Methods Mol Biol.
Dehdilani, N., Shamsasenjan, K., Movassaghpour, A., Akbarzadehlaleh, P., Amoughli Tabrizi, B., Parsa, H., and Sabagi, F. (2016). Improved Survival and Hematopoietic Differentiation of Murine Embryonic Stem Cells on Electrospun Polycaprolactone Nanofiber. Cell J 17, 629-638.
Duarte Campos, D. F., Blaeser, A., Buellesbach, K., Sen, K. S., Xun, W., Tillmann, W., and Fischer, H. (2016). Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering. Adv Healthc Mater.
Faghihi, F., Mirzaei, E., Ai, J., Lotfi, A., Sayahpour, F. A., Barough, S. E., and Joghataei, M. T. (2016). Differentiation Potential of Human Chorion-Derived Mesenchymal Stem Cells into Motor Neuron-Like Cells in Two- and Three-Dimensional Culture Systems. Mol Neurobiol 53, 1862-1872.
Follin, B., Juhl, M., Cohen, S., Perdersen, A. E., Kastrup, J., and Ekblond, A. (2016). Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture. Tissue Eng Part B Rev.
Gonzalez, S., Mei, H., Nakatsu, M. N., Baclagon, E. R., and Deng, S. X. (2016). A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16, 358-364.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Han, S., Wang, B., Li, X., Xiao, Z., Han, J., Zhao, Y., Fang, Y., Yin, Y., Chen, B., and Dai, J. (2016). Bone marrow-derived mesenchymal stem cells in three-dimensional culture promote neuronal regeneration by neurotrophic protection and immunomodulation. J Biomed Mater Res A.
He, P., Fu, J., and Wang, D. A. (2016). Murine pluripotent stem cells derived scaffold-free cartilage grafts from a micro-cavitary hydrogel platform. Acta Biomater.
Huang, Z., Zhen, Y., Yin, W., Ma, Z., and Zhang, L. (2016). Shh promotes sweat gland cell maturation in three-dimensional culture. Cell Tissue Bank.
Kang, L. H., Armstrong, P. A., Lee, L. J., Duan, B., Kang, K. H., and Butcher, J. T. (2016). Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels. Ann Biomed Eng.
Kato, T., Oka, K., Nakamura, T., and Ito, A. (2016). Decreased expression of Met during differentiation in rat lung. Eur J Histochem 60, 2575.
Katti, K. S., Molla, S., Karandish, F., Haldar, M. K., Mallik, S., and Katti, D. R. (2016). Sequential culture on biomimetic nanoclay scaffolds forms three dimensional tumoroids. J Biomed Mater Res A.
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Li, C. W., Pan, W. T., Ju, J. C., and Wang, G. J. (2016). An endothelial cultured condition medium embedded porous PLGA scaffold for the enhancement of mouse embryonic stem cell differentiation. Biomed Mater 11, 025015.
Lolli, A., Narcisi, R., Lambertini, E., Penolazzi, L., Angelozzi, M., Kops, N., Gasparini, S., van Osch, G. J., and Piva, R. (2016). Silencing of anti-chondrogenic microRNA-221 in human mesenchymal stem cells promotes cartilage repair in vivo. Stem Cells.
Medda, X., Mertens, L., Versweyveld, S., Diels, A., Barnham, L., Bretteville, A., Buist, A., Verheyen, A., Royaux, I., Ebneth, A., and Cabrera-Socorro, A. (2016). Development of a Scalable, High-Throughput-Compatible Assay to Detect Tau Aggregates Using iPSC-Derived Cortical Neurons Maintained in a Three-Dimensional Culture Format. J Biomol Screen.
Nemati, S., Abbasalizadeh, S., and Baharvand, H. (2016). Scalable Expansion of Human Pluripotent Stem Cell-Derived Neural Progenitors in Stirred Suspension Bioreactor Under Xeno-free Condition. Methods Mol Biol.
Nowotny, J., Aibibu, D., Farack, J., Nimtschke, U., Hild, M., Gelinsky, M., Kasten, P., and Cherif, C. (2016). Novel fiber-based pure chitosan scaffold for tendon augmentation: biomechanical and cell biological evaluation. J Biomater Sci Polym Ed, 1-20.
Oberbauer, E., Steffenhagen, C., Feichtinger, G., Hildner, F., Hacobian, A., Danzer, M., Gabriel, C., Redl, H., and Wolbank, S. (2016). A luciferase-based quick potency assay to predict chondrogenic differentiation. Tissue Eng Part C Methods.
Otani, T., Marchetto, M. C., Gage, F. H., Simons, B. D., and Livesey, F. J. (2016). 2D and 3D Stem Cell Models of Primate Cortical Development Identify Species-Specific Differences in Progenitor Behavior Contributing to Brain Size. Cell Stem Cell 18, 467-480.
Pouliot, R. A., Link, P. A., Mikhaiel, N. S., Schneck, M. B., Valentine, M. S., Kamga Gninzeko, F. J., Herbert, J. A., Sakagami, M., and Heise, R. L. (2016). Development and characterization of a naturally derived lung extracellular matrix hydrogel. J Biomed Mater Res A.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Raghothaman, D., Leong, M. F., Lim, T. C., Wan, A. C., Ser, Z., Lee, E. H., and Yang, Z. (2016). Cell type dependent morphological adaptation in polyelectrolyte hydrogels governs chondrogenic fate. Biomed Mater 11, 025013.
Relier, S., Yazdani, L., Ayad, O., Choquet, A., Bourgaux, J. F., Prudhomme, M., Pannequin, J., Macari, F., and David, A. (2016). Antibiotics inhibit sphere-forming ability in suspension culture. Cancer Cell Int 16, 6.
Robinson, S. T., Douglas, A. M., Chadid, T., Kuo, K., Rajabalan, A., Li, H., Copland, I. B., Barker, T. H., Galipeau, J., and Brewster, L. P. (2016). A Novel Platelet Lysate Hydrogel for Endothelial Cell and Mesenchymal Stem Cell-Directed Neovascularization. Acta Biomater.
Scioli, M. G., Bielli, A., Gentile, P., Cervelli, V., and Orlandi, A. (2016). Combined treatment with platelet-rich plasma and insulin favours chondrogenic and osteogenic differentiation of human adipose-derived stem cells in three-dimensional collagen scaffolds. J Tissue Eng Regen Med.
Simao, D., Arez, F., Terasso, A. P., Pinto, C., Sousa, M. F., Brito, C., and Alves, P. M. (2016). Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells. Methods Mol Biol.
Sokol, E. S., Miller, D. H., Breggia, A., Spencer, K. C., Arendt, L. M., and Gupta, P. B. (2016). Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Breast Cancer Res 18, 19.
Weigand, A., Boos, A. M., Tasbihi, K., Beier, J. P., Dalton, P. D., Schrauder, M., Horch, R. E., Beckmann, M. W., Strissel, P. L., and Strick, R. (2016). Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells. Breast Cancer Res 18, 32.
Wu, Y., Sun, J., George, J., Liu, Q., Ye, H., Ge, D., Liu, Y., Zhang, Y., Cui, Z., and Li, Z. (2016). Study of Neuroprotective Function of Ginkgo biloba Extract (EGb761) Derived-flavonoid Monomers Using a Three-dimensional Stem Cell-derived Neural Model. Biotechnol Prog.
Xu, Y., Shi, T., Xu, A., and Zhang, L. (2016). 3D spheroid culture enhances survival and therapeutic capacities of MSCs injected into ischemic kidney. J Cell Mol Med.
Ye, J., Wang, J., Zhu, Y., Wei, Q., Wang, X., Yang, J., Tang, S., Liu, H., Fan, J., Zhang, F., et al. (2016). A thermoresponsive polydiolcitrate-gelatin scaffold and delivery system mediates effective bone formation from BMP9-transduced mesenchymal stem cells. Biomed Mater 11, 025021.
Zhang, X., Hu, M. G., Pan, K., Li, C. H., and Liu, R. (2016). 3D Spheroid Culture Enhances the Expression of Antifibrotic Factors in Human Adipose-Derived MSCs and Improves Their Therapeutic Effects on Hepatic Fibrosis. Stem Cells Int 2016, 4626073.
Stromal Cells
Bellotti, C., Duchi, S., Bevilacqua, A., Lucarelli, E., and Piccinini, F. (2016). Long term morphological characterization of mesenchymal stromal cells 3D spheroids built with a rapid method based on entry-level equipment. Cytotechnology.
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Follin, B., Juhl, M., Cohen, S., Perdersen, A. E., Kastrup, J., and Ekblond, A. (2016). Increased Paracrine Immunomodulatory Potential of Mesenchymal Stromal Cells in Three-Dimensional Culture. Tissue Eng Part B Rev.
Gonzalez, S., Mei, H., Nakatsu, M. N., Baclagon, E. R., and Deng, S. X. (2016). A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells. Stem Cell Res 16, 358-364.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Keely, P., and Nain, A. (2015). Capturing relevant extracellular matrices for investigating cell migration. F1000Res 4.
Lo, Y. P., Liu, Y. S., Rimando, M. G., Ho, J. H., Lin, K. H., and Lee, O. K. (2016). Three-dimensional spherical spatial boundary conditions differentially regulate osteogenic differentiation of mesenchymal stromal cells. Sci Rep 6, 21253.
Lolli, A., Narcisi, R., Lambertini, E., Penolazzi, L., Angelozzi, M., Kops, N., Gasparini, S., van Osch, G. J., and Piva, R. (2016). Silencing of anti-chondrogenic microRNA-221 in human mesenchymal stem cells promotes cartilage repair in vivo. Stem Cells.
Niu, C., Chauhan, U., Gargus, M., and Shaker, A. (2016). Generation and Characterization of an Immortalized Human Esophageal Myofibroblast Line. PLoS One 11, e0153185.
Oldenburg, A. L., Yu, X., Gilliss, T., Alabi, O., Taylor, R. M., 2nd, and Troester, M. A. (2015). Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy. Optica 2, 877-885.
Parvizi, M., Bolhuis-Versteeg, L. A., Poot, A. A., and Harmsen, M. C. (2016). Efficient generation of smooth muscle cells from adipose-derived stromal cells by 3D mechanical stimulation can substitute the use of growth factors in vascular tissue engineering. Biotechnol J.
Ramakrishnan, V. M., Tien, K. T., McKinley, T. R., Bocard, B. R., McCurry, T. M., Williams, S. K., Hoying, J. B., and Boyd, N. L. (2016). Wnt5a Regulates the Assembly of Human Adipose Derived Stromal Vascular Fraction-Derived Microvasculatures. PLoS One 11, e0151402.
Sokol, E. S., Miller, D. H., Breggia, A., Spencer, K. C., Arendt, L. M., and Gupta, P. B. (2016). Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Breast Cancer Res 18, 19.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Tutak, W., Jyotsnendu, G., Bajcsy, P., and Simon, C. G., Jr. (2016). Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. J Biomed Mater Res B Appl Biomater.
Xie, Q., Xie, J., Zhong, J., Cun, X., Lin, S., Lin, Y., and Cai, X. (2016). Hypoxia enhances angiogenesis in an adipose-derived stromal cell/endothelial cell co-culture 3D gel model. Cell Prolif.
Yassin, M. A., Leknes, K. N., Sun, Y., Lie, S. A., Finne-Wistrand, A., and Mustafa, K. (2016). Surfactant tuning of hydrophilicity of porous degradable copolymer scaffolds promotes cellular proliferation and enhances bone formation. J Biomed Mater Res A.
Yu, T., Guo, Z., Fan, H., Song, J., Liu, Y., Gao, Z., and Wang, Q. (2016). Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device. Oncotarget.
Zheng, Y., Sun, Y., Yu, X., Shao, Y., Zhang, P., Dai, G., and Fu, J. (2016). Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis. Adv Healthc Mater.
Plant Cells
Corbin, J. M., Hashimoto, B. I., Karuppanan, K., Kyser, Z. R., Wu, L., Roberts, B. A., Noe, A. R., Rodriguez, R. L., McDonald, K. A., and Nandi, S. (2016). Semicontinuous Bioreactor Production of Recombinant Butyrylcholinesterase in Transgenic Rice Cell Suspension Cultures. Front Plant Sci 7, 412.
Luo, C. J., Wightman, R., Meyerowitz, E., and Smoukov, S. K. (2015). A 3-dimensional fibre scaffold as an investigative tool for studying the morphogenesis of isolated plant pells. BMC Plant Biol 15, 211.
Cancer/Tumor
Akasov, R., Zaytseva-Zotova, D., Burov, S., Leko, M., Dontenwill, M., Chiper, M., Vandamme, T., and Markvicheva, E. (2016). Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro. Int J Pharm.
Albritton, J. L., Roybal, J. D., Paulsen, S. J., Calafat, N., Flores-Zaher, J. A., Farach-Carson, M. C., Gibbons, D. L., and Miller, J. S. (2016). Ultrahigh-throughput Generation and Characterization of Cellular Aggregates in Laser-ablated Microwells of Poly(dimethylsiloxane). RSC Adv 6, 8980-8991.
Alonso-Nocelo, M., Abuin, C., Lopez-Lopez, R., and de la Fuente, M. (2016). Development and characterization of a three-dimensional co-culture model of tumor T cell infiltration. Biofabrication 8, 025002.
Arpin, C. C., Mac, S., Jiang, Y., Cheng, H., Grimard, M., Page, B. D., Kamocka, M. M., Haftchenary, S., Su, H., Ball, D. P., et al. (2016). Applying Small Molecule Signal Transducer and Activator of Trancription-3 (STAT3) Protein Inhibitors as Pancreatic Cancer Therapeutics. Mol Cancer Ther.
Boyce, M. W., Kenney, R. M., Truong, A. S., and Lockett, M. R. (2016). Quantifying oxygen in paper-based cell cultures with luminescent thin film sensors. Anal Bioanal Chem 408, 2985-2992.
Cardoso, T. C., Sakamoto, S. S., Stockmann, D., Souza, T. F., Ferreira, H. L., Gameiro, R., Vieira, F. V., Louzada, M. J., Andrade, A. L., and Flores, E. F. (2016). A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators. Vet Comp Oncol.
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Emura, M., and Aufderheide, M. (2016). Challenge for 3D culture technology: Application in carcinogenesis studies with human airway epithelial cells. Exp Toxicol Pathol.
Fischer, K. M., Morgan, K. Y., Hearon, K., Sklaviadis, D., Tochka, Z. L., Fenton, O. S., Anderson, D. G., Langer, R., and Freed, L. E. (2016). Poly(Limonene Thioether) Scaffold for Tissue Engineering. Adv Healthc Mater.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Itou, J., Tanaka, S., Li, W., Matsumoto, Y., Sato, F., and Toi, M. (2015). Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells. Data Brief 5, 429-433.
Jobe, N. P., Rosel, D., Dvorankova, B., Kodet, O., Lacina, L., Mateu, R., Smetana, K., and Brabek, J. (2016). Simultaneous blocking of IL-6 and IL-8 is sufficient to fully inhibit CAF-induced human melanoma cell invasiveness. Histochem Cell Biol.
Katt, M. E., Placone, A. L., Wong, A. D., Xu, Z. S., and Searson, P. C. (2016). In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform. Front Bioeng Biotechnol 4, 12.
Katti, K. S., Molla, S., Karandish, F., Haldar, M. K., Mallik, S., and Katti, D. R. (2016). Sequential culture on biomimetic nanoclay scaffolds forms three dimensional tumoroids. J Biomed Mater Res A.
Keely, P., and Nain, A. (2015). Capturing relevant extracellular matrices for investigating cell migration. F1000Res 4.
Kim, J., and Tanner, K. (2016). Three-Dimensional Patterning of the ECM Microenvironment Using Magnetic Nanoparticle Self Assembly. Curr Protoc Cell Biol 70, 25.23.21-25.23.14.
Koeck, S., Zwierzina, M., Huber, J. M., Bitsche, M., Lorenz, E., Gamerith, G., Dudas, J., Kelm, J. M., Zwierzina, H., and Amann, A. (2016). Infiltration of lymphocyte subpopulations into cancer microtissues as a tool for the exploration of immunomodulatory agents and biomarkers. Immunobiology 221, 604-617.
Lapa, G. B., Tsunoda, T., Shirasawa, S., Baryshnikova, M. A., Evseev, G. G., Afanasyeva, D. A., and Chigorina, E. A. (2016). Synthesis of New Congeners of 1-methyl-3-aminoisoquinolines, Evaluation of Their Cytotoxic Activity, In Silico and In Vitro Study of Their Molecular Targets as PDE4B. Chem Biol Drug Des 87, 575-582.
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Liu, H., Liu, J., Qi, C., Fang, Y., Zhang, L., Zhuo, R., and Jiang, X. (2016). Thermosensitive injectable in-situ forming carboxymethyl chitin hydrogel for three-dimensional cell culture. Acta Biomater 35, 228-237.
Loessner, D., Meinert, C., Kaemmerer, E., Martine, L. C., Yue, K., Levett, P. A., Klein, T. J., Melchels, F. P., Khademhosseini, A., and Hutmacher, D. W. (2016). Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. Nat Protoc 11, 727-746.
Meenach, S. A., Tsoras, A. N., McGarry, R. C., Mansour, H. M., Hilt, J. Z., and Anderson, K. W. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. Int J Oncol 48, 1701-1709.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Oldenburg, A. L., Yu, X., Gilliss, T., Alabi, O., Taylor, R. M., 2nd, and Troester, M. A. (2015). Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy. Optica 2, 877-885.
Pati, F., Gantelius, J., and Svahn, H. A. (2016). 3D Bioprinting of Tissue/Organ Models. Angew Chem Int Ed Engl.
Patra, B., Peng, C. C., Liao, W. H., Lee, C. H., and Tung, Y. C. (2016). Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device. Sci Rep 6, 21061.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Relier, S., Yazdani, L., Ayad, O., Choquet, A., Bourgaux, J. F., Prudhomme, M., Pannequin, J., Macari, F., and David, A. (2016). Antibiotics inhibit sphere-forming ability in suspension culture. Cancer Cell Int 16, 6.
Robinson, S. T., Douglas, A. M., Chadid, T., Kuo, K., Rajabalan, A., Li, H., Copland, I. B., Barker, T. H., Galipeau, J., and Brewster, L. P. (2016). A Novel Platelet Lysate Hydrogel for Endothelial Cell and Mesenchymal Stem Cell-Directed Neovascularization. Acta Biomater.
Ryan, S. L., Baird, A. M., Vaz, G., Urquhart, A. J., Senge, M., Richard, D. J., O'Byrne, K. J., and Davies, A. M. (2016). Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture. Assay Drug Dev Technol 14, 19-28.
Sarkar, J., and Kumar, A. (2016). Thermo-responsive polymer aided spheroid culture in cryogel based platform for high throughput drug screening. Analyst.
Sato, K., Choyke, P. L., and Hisataka, K. (2016). Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique. J Vis Exp.
Schmidt, M., Scholz, C. J., Polednik, C., and Roller, J. (2016). Spheroid-based 3-dimensional culture models: Gene expression and functionality in head and neck cancer. Oncol Rep 35, 2431-2440.
Sims, L. B., Curtis, L. T., Frieboes, H. B., and Steinbach-Rankins, J. M. (2016). Enhanced uptake and transport of PLGA-modified nanoparticles in cervical cancer. J Nanobiotechnology 14, 33.
Sokol, E. S., Miller, D. H., Breggia, A., Spencer, K. C., Arendt, L. M., and Gupta, P. B. (2016). Growth of human breast tissues from patient cells in 3D hydrogel scaffolds. Breast Cancer Res 18, 19.
Soon, C. F., Thong, K. T., Tee, K. S., Ismail, A. B., Denyer, M., Ahmad, M. K., Kong, Y. H., Vyomesh, P., and Cheong, S. C. (2016). A scaffoldless technique for self-generation of three-dimensional keratinospheroids on liquid crystal surfaces. Biotech Histochem, 1-13.
Taubenberger, A. V., Bray, L. J., Haller, B., Shaposhnykov, A., Binner, M., Freudenberg, U., Guck, J., and Werner, C. (2016). 3D extracellular matrix interactions modulate tumour cell growth, invasion and angiogenesis in engineered tumour microenvironments. Acta Biomater.
Wang, J. Z., Zhu, Y. X., Ma, H. C., Chen, S. N., Chao, J. Y., Ruan, W. D., Wang, D., Du, F. G., and Meng, Y. Z. (2016). Developing multi-cellular tumor spheroid model (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. Mater Sci Eng C Mater Biol Appl 62, 215-225.
Weigand, A., Boos, A. M., Tasbihi, K., Beier, J. P., Dalton, P. D., Schrauder, M., Horch, R. E., Beckmann, M. W., Strissel, P. L., and Strick, R. (2016). Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells. Breast Cancer Res 18, 32.
Xu, H., Liu, W., Zhang, X. Z., Hou, L., Lu, Y. J., Chen, P. P., Zhang, C., Feng, D., Kong, L., and Wang, X. L. (2016). [Development of three-dimensional breast cancer cell culture drug resistance model]. Sheng Li Xue Bao 68, 179-184.
Yoshii, Y., Furukawa, T., Aoyama, H., Adachi, N., Zhang, M. R., Wakizaka, H., Fujibayashi, Y., and Saga, T. (2016). Regorafenib as a potential adjuvant chemotherapy agent in disseminated small colon cancer: Drug selection outcome of a novel screening system using nanoimprinting 3-dimensional culture with HCT116-RFP cells. Int J Oncol 48, 1477-1484.
Yu, T., Guo, Z., Fan, H., Song, J., Liu, Y., Gao, Z., and Wang, Q. (2016). Cancer-associated fibroblasts promote non-small cell lung cancer cell invasion by upregulation of glucose-regulated protein 78 (GRP78) expression in an integrated bionic microfluidic device. Oncotarget.
Zheng, Y., Sun, Y., Yu, X., Shao, Y., Zhang, P., Dai, G., and Fu, J. (2016). Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis. Adv Healthc Mater.
Zhu, W., Ma, X., Gou, M., Mei, D., Zhang, K., and Chen, S. (2016). 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol 40, 103-112.
Screening
Akasov, R., Zaytseva-Zotova, D., Burov, S., Leko, M., Dontenwill, M., Chiper, M., Vandamme, T., and Markvicheva, E. (2016). Formation of multicellular tumor spheroids induced by cyclic RGD-peptides and use for anticancer drug testing in vitro. Int J Pharm.
Alonso-Nocelo, M., Abuin, C., Lopez-Lopez, R., and de la Fuente, M. (2016). Development and characterization of a three-dimensional co-culture model of tumor T cell infiltration. Biofabrication 8, 025002.
Asthana, A., and Kisaalita, W. S. (2016). Molecular basis for cytokine biomarkers of complex 3D microtissue physiology in vitro. Drug Discov Today.
Barata, D., van Blitterswijk, C., and Habibovic, P. (2016). High-throughput screening approaches and combinatorial development of biomaterials using microfluidics. Acta Biomater 34, 1-20.
Bilgin, C. C., Fontenay, G., Cheng, Q., Chang, H., Han, J., and Parvin, B. (2016). BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models. PLoS One 11, e0148379.
Casey, A., Gargotti, M., Bonnier, F., and Byrne, H. J. (2016). Chemotherapeutic efficiency of drugs in vitro: Comparison of doxorubicin exposure in 3D and 2D culture matrices. Toxicol In Vitro 33, 99-104.
Chi, C. W., Ahmed, A. R., Dereli-Korkut, Z., and Wang, S. (2016). Microfluidic cell chips for high throughput drug screening. Bioanalysis, 0.
Del Bufalo, F., Manzo, T., Hoyos, V., Yagyu, S., Caruana, I., Jacot, J., Benavides, O., Rosen, D., and Brenner, M. K. (2016). 3D modeling of human cancer: A PEG-fibrin hydrogel system to study the role of tumor microenvironment and recapitulate the in vivo effect of oncolytic adenovirus. Biomaterials 84, 76-85.
Ham, S. L., Joshi, R., Thakuri, P. S., and Tavana, H. (2016). Liquid-based three-dimensional tumor models for cancer research and drug discovery. Exp Biol Med (Maywood).
Itou, J., Tanaka, S., Li, W., Matsumoto, Y., Sato, F., and Toi, M. (2015). Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells. Data Brief 5, 429-433.
Katt, M. E., Placone, A. L., Wong, A. D., Xu, Z. S., and Searson, P. C. (2016). In Vitro Tumor Models: Advantages, Disadvantages, Variables, and Selecting the Right Platform. Front Bioeng Biotechnol 4, 12.
Koeck, S., Zwierzina, M., Huber, J. M., Bitsche, M., Lorenz, E., Gamerith, G., Dudas, J., Kelm, J. M., Zwierzina, H., and Amann, A. (2016). Infiltration of lymphocyte subpopulations into cancer microtissues as a tool for the exploration of immunomodulatory agents and biomarkers. Immunobiology 221, 604-617.
Lapa, G. B., Tsunoda, T., Shirasawa, S., Baryshnikova, M. A., Evseev, G. G., Afanasyeva, D. A., and Chigorina, E. A. (2016). Synthesis of New Congeners of 1-methyl-3-aminoisoquinolines, Evaluation of Their Cytotoxic Activity, In Silico and In Vitro Study of Their Molecular Targets as PDE4B. Chem Biol Drug Des 87, 575-582.
Laundos, T. L., Silva, J., Assuncao, M., Quelhas, P., Monteiro, C., Oliveira, C., Oliveira, M. J., Pego, A. P., and Amaral, I. F. (2016). Rotary orbital suspension culture of embryonic stem cell-derived neural stem/progenitor cells: impact of hydrodynamic culture on aggregate yield, morphology and cell phenotype. J Tissue Eng Regen Med.
Medda, X., Mertens, L., Versweyveld, S., Diels, A., Barnham, L., Bretteville, A., Buist, A., Verheyen, A., Royaux, I., Ebneth, A., and Cabrera-Socorro, A. (2016). Development of a Scalable, High-Throughput-Compatible Assay to Detect Tau Aggregates Using iPSC-Derived Cortical Neurons Maintained in a Three-Dimensional Culture Format. J Biomol Screen.
Meenach, S. A., Tsoras, A. N., McGarry, R. C., Mansour, H. M., Hilt, J. Z., and Anderson, K. W. (2016). Development of three-dimensional lung multicellular spheroids in air- and liquid-interface culture for the evaluation of anticancer therapeutics. Int J Oncol 48, 1701-1709.
Nath, S., and Devi, G. R. (2016). Three-Dimensional Culture Systems in Cancer Research: Focus on Tumor Spheroid Model. Pharmacol Ther.
Pati, F., Gantelius, J., and Svahn, H. A. (2016). 3D Bioprinting of Tissue/Organ Models. Angew Chem Int Ed Engl.
Patra, B., Peng, C. C., Liao, W. H., Lee, C. H., and Tung, Y. C. (2016). Drug testing and flow cytometry analysis on a large number of uniform sized tumor spheroids using a microfluidic device. Sci Rep 6, 21061.
Qiao, S. P., Zhao, Y. F., Li, C. F., Yin, Y. B., Meng, Q. Y., Lin, F. H., Liu, Y., Hou, X. L., Guo, K., Chen, X. B., and Tian, W. M. (2016). An Alginate-based Platform for Cancer Stem Cell Research. Acta Biomater.
Ryan, S. L., Baird, A. M., Vaz, G., Urquhart, A. J., Senge, M., Richard, D. J., O'Byrne, K. J., and Davies, A. M. (2016). Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture. Assay Drug Dev Technol 14, 19-28.
Sarkar, J., and Kumar, A. (2016). Thermo-responsive polymer aided spheroid culture in cryogel based platform for high throughput drug screening. Analyst.
Simao, D., Arez, F., Terasso, A. P., Pinto, C., Sousa, M. F., Brito, C., and Alves, P. M. (2016). Perfusion Stirred-Tank Bioreactors for 3D Differentiation of Human Neural Stem Cells. Methods Mol Biol.
Wang, J. Z., Zhu, Y. X., Ma, H. C., Chen, S. N., Chao, J. Y., Ruan, W. D., Wang, D., Du, F. G., and Meng, Y. Z. (2016). Developing multi-cellular tumor spheroid model (MCTS) in the chitosan/collagen/alginate (CCA) fibrous scaffold for anticancer drug screening. Mater Sci Eng C Mater Biol Appl 62, 215-225.
Yoshii, Y., Furukawa, T., Aoyama, H., Adachi, N., Zhang, M. R., Wakizaka, H., Fujibayashi, Y., and Saga, T. (2016). Regorafenib as a potential adjuvant chemotherapy agent in disseminated small colon cancer: Drug selection outcome of a novel screening system using nanoimprinting 3-dimensional culture with HCT116-RFP cells. Int J Oncol 48, 1477-1484.
3D Bioprinting
Alessandri, K., Feyeux, M., Gurchenkov, B., Delgado, C., Trushko, A., Krause, K. H., Vignjevic, D., Nassoy, P., and Roux, A. (2016). A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC). Lab Chip.
Duarte Campos, D. F., Blaeser, A., Buellesbach, K., Sen, K. S., Xun, W., Tillmann, W., and Fischer, H. (2016). Bioprinting Organotypic Hydrogels with Improved Mesenchymal Stem Cell Remodeling and Mineralization Properties for Bone Tissue Engineering. Adv Healthc Mater.
Kang, L. H., Armstrong, P. A., Lee, L. J., Duan, B., Kang, K. H., and Butcher, J. T. (2016). Optimizing Photo-Encapsulation Viability of Heart Valve Cell Types in 3D Printable Composite Hydrogels. Ann Biomed Eng.
Kingsley, D. M., Dias, A. D., and Corr, D. T. (2016). Microcapsules and 3D customizable shelled microenvironments from laser direct-written microbeads. Biotechnol Bioeng.
Lei, Y., and Ferdous, Z. (2016). Design Considerations and Challenges for Mechanical Stretch Bioreactors in Tissue Engineering. Biotechnol Prog.
Pati, F., Gantelius, J., and Svahn, H. A. (2016). 3D Bioprinting of Tissue/Organ Models. Angew Chem Int Ed Engl.
Pinnock, C. B., Meier, E. M., Joshi, N. N., Wu, B., and Lam, M. T. (2016). Customizable engineered blood vessels using 3D printed inserts. Methods 99, 20-27.
Suntornnond, R., An, J., Tijore, A., Leong, K. F., Chua, C. K., and Tan, L. P. (2016). A Solvent-Free Surface Suspension Melt Technique for Making Biodegradable PCL Membrane Scaffolds for Tissue Engineering Applications. Molecules 21.
Tan, Y. J., Yeong, W. Y., Tan, X., An, J., Chian, K. S., and Leong, K. F. (2016). Characterization, mechanical behavior and in vitro evaluation of a melt-drawn scaffold for esophageal tissue engineering. J Mech Behav Biomed Mater 57, 246-259.
Wang, H., Wu, G., Zhang, J., Zhou, K., Yin, B., Su, X., Qiu, G., Yang, G., Zhang, X., Zhou, G., and Wu, Z. (2016). Osteogenic effect of controlled released rhBMP-2 in 3D printed porous hydroxyapatite scaffold. Colloids Surf B Biointerfaces 141, 491-498.
Zhu, W., Ma, X., Gou, M., Mei, D., Zhang, K., and Chen, S. (2016). 3D printing of functional biomaterials for tissue engineering. Curr Opin Biotechnol 40, 103-112.
Imaging
Bellotti, C., Duchi, S., Bevilacqua, A., Lucarelli, E., and Piccinini, F. (2016). Long term morphological characterization of mesenchymal stromal cells 3D spheroids built with a rapid method based on entry-level equipment. Cytotechnology.
Bilgin, C. C., Fontenay, G., Cheng, Q., Chang, H., Han, J., and Parvin, B. (2016). BioSig3D: High Content Screening of Three-Dimensional Cell Culture Models. PLoS One 11, e0148379.
Boyce, M. W., Kenney, R. M., Truong, A. S., and Lockett, M. R. (2016). Quantifying oxygen in paper-based cell cultures with luminescent thin film sensors. Anal Bioanal Chem 408, 2985-2992.
Christoffersson, J., Bergstrom, G., Schwanke, K., Kempf, H., Zweigerdt, R., and Mandenius, C. F. (2016). A Microfluidic Bioreactor for Toxicity Testing of Stem Cell Derived 3D Cardiac Bodies. Methods Mol Biol.
Duan, B., Yin, Z., Hockaday Kang, L., Magin, R. L., and Butcher, J. T. (2016). Active tissue stiffness modulation controls valve interstitial cell phenotype and osteogenic potential in 3D culture. Acta Biomater.
Itou, J., Tanaka, S., Li, W., Matsumoto, Y., Sato, F., and Toi, M. (2015). Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells. Data Brief 5, 429-433.
O'Leary, C., Cavanagh, B., Unger, R. E., Kirkpatrick, C. J., O'Dea, S., O'Brien, F. J., and Cryan, S. A. (2016). The development of a tissue-engineered tracheobronchial epithelial model using a bilayered collagen-hyaluronate scaffold. Biomaterials 85, 111-127.
Oldenburg, A. L., Yu, X., Gilliss, T., Alabi, O., Taylor, R. M., 2nd, and Troester, M. A. (2015). Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy. Optica 2, 877-885.
Robinson, S. T., Douglas, A. M., Chadid, T., Kuo, K., Rajabalan, A., Li, H., Copland, I. B., Barker, T. H., Galipeau, J., and Brewster, L. P. (2016). A Novel Platelet Lysate Hydrogel for Endothelial Cell and Mesenchymal Stem Cell-Directed Neovascularization. Acta Biomater.
Ryan, S. L., Baird, A. M., Vaz, G., Urquhart, A. J., Senge, M., Richard, D. J., O'Byrne, K. J., and Davies, A. M. (2016). Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture. Assay Drug Dev Technol 14, 19-28.
Sato, K., Choyke, P. L., and Hisataka, K. (2016). Selective Cell Elimination from Mixed 3D Culture Using a Near Infrared Photoimmunotherapy Technique. J Vis Exp.
Shalaly, N. D., Ria, M., Johansson, U., Avall, K., Berggren, P. O., and Hedhammar, M. (2016). Silk matrices promote formation of insulin-secreting islet-like clusters. Biomaterials 90, 50-61.
Tutak, W., Jyotsnendu, G., Bajcsy, P., and Simon, C. G., Jr. (2016). Nanofiber scaffolds influence organelle structure and function in bone marrow stromal cells. J Biomed Mater Res B Appl Biomater.
Weber, L., Langer, M., Tavella, S., Ruggiu, A., and Peyrin, F. (2016). Quantitative evaluation of regularized phase retrieval algorithms on bone scaffolds seeded with bone cells. Phys Med Biol 61, N215-n231.
Yoon, H. I., Yhee, J. Y., Na, J. H., Lee, S., Lee, H., Kang, S. W., Chang, H., Ryu, J. H., Kwon, I. C., Cho, Y. W., and Kim, K. (2016). Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo. Bioconjug Chem 27, 927-936.
Yoshii, Y., Furukawa, T., Aoyama, H., Adachi, N., Zhang, M. R., Wakizaka, H., Fujibayashi, Y., and Saga, T. (2016). Regorafenib as a potential adjuvant chemotherapy agent in disseminated small colon cancer: Drug selection outcome of a novel screening system using nanoimprinting 3-dimensional culture with HCT116-RFP cells. Int J Oncol 48, 1477-1484.
Zanotelli, M. R., Ardalani, H., Zhang, J., Hou, Z., Nguyen, E. H., Swanson, S., Nguyen, B. K., Bolin, J., Elwell, A., Bischel, L. L., et al. (2016). Stable engineered vascular networks from human induced pluripotent stem cell-derived endothelial cells cultured in synthetic hydrogels. Acta Biomater.
Quantitation
Langan, L. M., Dodd, N. J., Owen, S. F., Purcell, W. M., Jackson, S. K., and Jha, A. N. (2016). Direct Measurements of Oxygen Gradients in Spheroid Culture System Using Electron Parametric Resonance Oximetry. PLoS One 11, e0149492.
Paulose, T., Montevil, M., Speroni, L., Cerruti, F., Sonnenschein, C., and Soto, A. M. (2016). SAMA: A Method for 3D Morphological Analysis. PLoS One 11, e0153022.
3D in Meetings
3D Cell Culture 2016 (3DCC 2016)
Date: 18th to 21st April 2016
Location: Freiburg, Germany
Organized by: DECHEMA, Gesellschaft für Chemische Technik und Biotechnologie e.V
Event Website: http://www.dechema.de/3DCC2016.html
3rd 3D Models & Drug Screening Conference
Date: 11th to 12th May 2016
Location: Berlin, Germany
Website https://www.gtcbio.com/conferences/3d-models-drug-screening-overview
Contact Person - Kristen Starkey
Event enquiries email address - infogtcbio@gtcbio.com
Deadline for abstracts/proposals: 2016-04-11
Organized by: GTCbio
Healthcare of the Future: Analytics, Wearables, 3D Printing and Digital Innovations conference 2016
Date: 15th to 17th June 2016
Location: Sydney, Australia
Website: http://claridenglobal.com/conference/au-digitalhealthcare2016/
Contact person: Sherin Edward
Organized by: Clariden Global International Limited
Organ-on-a-Chip World Congress & 3D-Culture 2016
Date: 7th July to 8th July 2016
Location: Boston, USA
https://selectbiosciences.com/conferences/index.aspx?conf=OOACWC2016
Bioprinting & 3D Printing in the Life Sciences
Date: 21st to 22nd July 2016
Location: Singapore
Website: http://selectbiosciences.com/conferences/index.aspx?conf=BIO3D
Contact person: Paul Raggett
2nd EACR Conference on Goodbye Flat Biology: Models, Mechanisms and Microenvironment
Date: 2nd to 5th October 2016
Location: Berlin, Germany
Website: http://www.eacr.org/goodbyeflatbiology2016/index.php
Contact person: Roger Doxat-Pratt
The meeting should be of interest to all those who use cancer cell lines, patient-derived tissue samples or primary cultures in vitro for the study of tumour biology, bioengineering and biochemistry, drug target validation, etc.
Organized by: European Association for Cancer Research
Deadline for abstracts/proposals: 20th June 2016
Organoids: Modelling Organ Development and Disease in 3D Culture
Date: 12th to 15th October 2016
Location: EMBL Heidelberg, Germany
http://www.embo-embl-symposia.org/symposia/2016/EES16-07/index.html
Organized by: EMBL
3D Bioprinting
Date: 13th October to 14th October 2016
Location: Cambridge, UK
https://selectbiosciences.com/conferences/index.aspx?conf=BPEURO2016
Organized by: SELECTBIO